激光生物学报2018，Vol.27Issue(2)：166-174,182,10.DOI:10.3969/j.issn.1007-7146.2018.02.010
抗人甲状腺球蛋白单克隆抗体的制备
Preparation of Monoclonal Antibody against Human Thyroglobulin
摘要
Abstract
Objective: To prepare monoclonal antibody against human thyroglobulin and establish a highly sensitive method to detect human thyroglobulin. Methods:BALB/c mice were subcutaneously immunized by native human thyro-globulin, the splenocytes of immunized mice were fused with SP2/0 myeloma cells by a routine method. The hybridoma cells secreting specific antibody were detected. The stability of the obtained hybridoma cells and the specificity of anti-thyroglobulin monoclonal antibody the hybridoma cells secreted were identified. Based on all above, the double antibody sandwich ELISA method was established to detect thyroglobulin. Results: Seven hybridoma cell lines which could se-crete monoclonal antibody stably were obtained, and ELISA indicated the antibodies had a good specificity and sensitivity to Tg antigen. A sandwich ELISA kit for detecting human thyroglobulin was established, and its sensitivity reached to 1 ng/mL. Conclusion: The anti-thyroglobulin monoclonal antibodies were prepared and a sandwich ELISA kit for the de-tection of human thyroglobulin was established successfully, which would provide a raw material for the development of Tg rapid diagnostic kits.关键词
单克隆抗体/甲状腺球蛋白/纯化/酶联免疫吸附实验Key words
monoclonal antibody/thyroglobulin/purification/enzyme-linked immunosorbent assay分类
医药卫生引用本文复制引用
靳晓瑞,吴意,廖旻晶,邱义兰,唐娜,曾林秀,王平,郭向荣,刘如石..抗人甲状腺球蛋白单克隆抗体的制备[J].激光生物学报,2018,27(2):166-174,182,10.基金项目
省自然科学基金(2018JJ2226) （2018JJ2226）
湖南省重点研发计划(2015SK20332) （2015SK20332）
