吉林大学学报（理学版）2018，Vol.56Issue(3)：732-737,6.DOI:10.13413/j.cnki.jdxblxb.2018.03.44
邻香草醛缩壳寡糖席夫碱及其Cu(Ⅱ)配合物与DNA的相互作用
Interaction of Schiff Base Derived from Oligochitosan and o-Vanillin and Its Cu (Ⅱ ) Complex with DNA
摘要
Abstract
The natural polymer oligochitosan was modified using o-vanillin . By the excellent coordination properties of the Schiff base structure ,the copper complex of o-vanillic aldehyde modified oligochitosan was prepared .Cyclic voltammetry curves indicated that Schiff base-copper complexes possessed electrochemical activity ,and the reaction of central Cu2+ at glassy carbonelectrodes was controlled by diffusion process mechanism in the range of scan rate from 0.025—1.0 V/s . We investigated the mechanism of the interaction of Schiff base and Cu complex with DNA by using cyclic voltammetry ,ultraviolet spectrum ,viscosity and melting point method .The results show that ,after adding DNA , the oxidation peak current of the complex decreases and the peak potential moves weakly .VCOS-Cu13 ,VCOS-Cu31 and DNA can form complexes of molar ratio of 1 : 1 ,and the binding constants are 0 .78 × 104 ,0 .69 × 104 L/mol .The intensities of absorption peaks of complexes are reduced and the peak position appears red shift .The relative viscosity and melting point of DNA increase after adding the complex .There are interca lation action between different molar of complex and DN A .关键词
席夫碱/Cu(Ⅱ)配合物/结合DNAKey words
Schiff base/Cu (Ⅱ) complex/DNA binding分类
化学化工引用本文复制引用
冯小强,李小芳,朱元成..邻香草醛缩壳寡糖席夫碱及其Cu(Ⅱ)配合物与DNA的相互作用[J].吉林大学学报（理学版）,2018,56(3):732-737,6.基金项目
甘肃省青年科技基金(批准号:1606RJYE247)、甘肃省高等学校科研项目(批准号:2016B-071)、天水市科技支撑项目(批准号:2016)和天水师范学院中青年教师科研项目(批准号:TSA1508). （批准号:1606RJYE247）
