军事医学2018，Vol.42Issue(2)：101-104,4.DOI:10.7644/j.issn.1674-9960.2018.02.005
TFPR1通过激活树突状细胞并促进其成熟发挥佐剂功能
TFPR1 acts as a novel adjuvant by activating dendritic cells and promoting its matu-ration
摘要
Abstract
Objective To study the role of dendritic cells in the function of a recombinant protein TFPR 1 as an adjuvant .Methods Bone marrow cells were collected from four-to five-week-old male BALB/c mice under aseptic conditions, and cultured with complete RPMI 1640 containing rmGM-CSF and rmIL-4 for six days.TFPR1 was added on day 6, and cells were incubated for another 24 hours.LPS was used as positive control , while PBS as negative .The morphology of dendritic cells was observed under an optical microscope and laser confocal microscope , cell surface makers (CD40,CD80,CD86 and MHCⅡ)were detected with flow cytometry, and the cytokines in the supernatant were detected with ELISA.Results Compared with negative control ,dendritic cells incubated with TFPR1 for 24 hours were significantly different in morphology as was observed by optical and laser confocal microscopes , but were similar to positive control .Most of the dendritic cells treated with TFPR 1 showed less adherence and became round , whose podosomes became shorter , and even disappeared .Actin distribution changed from two poles of the cell to the membrane .CD40,CD80, CD86 and MHCⅡon the cell surface were up-regulated on stimulation by TFPR1,as was detected by FACS.These results showed that TFPR1 was capable of promoting dendritic cell maturation .ELISA showed dendritic cells treated with TFPR 1 secreted high levels of cytokines(IL-6, IL-8 and TNF-α).Conclusion TFPR1 is capable of promoting dendritic cell maturation , and activating cells to produce cytokines , indicating that dendritic cells can play an important role in the function of TFPR 1 as a novel ad-juvant .关键词
TFPR1/树突状细胞/佐剂/作用机制Key words
TFPR1/dendritic cell/adjuvant/mechanism分类
医药卫生引用本文复制引用
李悄,王月鹏,朱青,宁秀哲,孙维来,周育森,寇志华..TFPR1通过激活树突状细胞并促进其成熟发挥佐剂功能[J].军事医学,2018,42(2):101-104,4.基金项目
病原微生物生物安全国家重点实验室基金资助项目(SKPBS1416) （SKPBS1416）
