军事医学2018，Vol.42Issue(2)：105-109,5.DOI:10.7644/j.issn.1674-9960.2018.02.006
BTN3A3单克隆抗体的制备与鉴定
Preparation and identification of monoclonal antibody against BNTN3 A3
摘要
Abstract
Objective To prepare and identify monoclonal antibodies against BTN 3A3.Methods BALB/c mice were immunized with the BTN3A3 recombinant protein BTN3A3-mFc containing the mouse IgG2a Fc tag as an antigen, and hybridoma cells were obtained by cell fusion .BTN3A3 recombinant protein BTN3A3-hFc containing a human IgG1 Fc tag as a detection target was used to screen hybridoma cell lines secreting monoclonal antibodies against BTN 3A3.The above monoclonal antibodies were identified with Western blot and indirect immunofluorescence .Results Seven hybridoma cell lines that could stably secret BTN 3A3 monoclonal antibodies were obtained . ELISA, Western blot and indirect immunofluorescence showed that the above antibodies specifically recognized BTN 3A3.Conclusion BTN3A3 monoclonal antibodies that can be applied to ELISA , Western blot or immunofluorescence have been obtained , which can contribute to the functional study of BTN3A3.关键词
嗜乳脂蛋白家族/BTN3A3/单克隆抗体/杂交瘤/免疫球蛋白类Key words
butyrophilins/BTN3A3/monoclonal antibody/hybridomas/immunglobulins分类
生物科学引用本文复制引用
陆倩,柳迪,唐丽..BTN3A3单克隆抗体的制备与鉴定[J].军事医学,2018,42(2):105-109,5.基金项目
国家自然科学基金资助项目(31570901) （31570901）
