军事医学2018，Vol.42Issue(2)：110-113,4.DOI:10.7644/j.issn.1674-9960.2018.02.007
不同方法提取寡聚RNA-DNA杂合体效果比较
Comparison of methods for extraction of short RNA-DNA hybrids
摘要
Abstract
Objective To compare the effects of three different methods for extracting short RNA-DNA hybrids, including the TRI reagent method , the phenol saturated with water method and the phenol saturated with Tris buffer method in order to facilitate studies on the biological function of RNA-DNA hybrids .Methods Short RNA fragments modifiedwith FAM at the 5′end and those modified with Cy 5 at the 5′end were synthesized .RNA and DNA fragments were annealed to form RNA-DNA hybrids.They were extracted with the above-mentioned 3 methods respectively .The extracted products were analyzed with electrophoresis .Results and Conclusion Short RNA-DNA hybrids can be extracted by the phenol saturated with water method and by the phenol saturated with Tris buffer method .The results can help study the function of short RNA-DNA hybrids .关键词
RNA-DNA杂合体/酚/TRI/提取/基因表达调控Key words
RNA-DNA hybrid/phenol/TRI reagent/extraction/gene expression regulation分类
生物科学引用本文复制引用
薛勇,付汉江,葛常辉,李清,郑晓飞..不同方法提取寡聚RNA-DNA杂合体效果比较[J].军事医学,2018,42(2):110-113,4.基金项目
国家自然科学基金资助项目(31470782,91540202) （31470782,91540202）
国家重点研发计划资助项目(2016YFC1303604) （2016YFC1303604）
