军事医学2018，Vol.42Issue(2)：124-129,6.DOI:10.7644/j.issn.1674-9960.2018.02.010
金纳米星/胶原复合基质材料对新生大鼠心室肌细胞氧化应激损伤的影响
Effect of Au NanoStars/collagen matrix material on oxidative stress damage in ventricular myocytes of newborn rats
摘要
Abstract
Objective To verify antioxidation of Au NanoStars/collagen ( AuNSs/Col ) for ventricular myocytes of newborn rats(NRVMs) by in vitro studies.Methods (1)Different concentrations of AuNSs/Col composite materials were created.The optimum concentration of the material was selected by Live /dead staining and Cell Counting Kit-8 (CCK-8) and Col was used for subsequent experiments .( 2 ) NRVMs were randomly divided into Col group , AuNSs/Col group, H2O2-induced Col group, and H2O2-induced AuNSs/Col group.After 6 h treatment, apoptotic cell morphology and early cell apoptosis rate were observed with Annexin Ⅴ-FITC/propidium iodide ( PI)/4′,6-diamidino-2-phenylindole ( DAPI) and the expressions of apoptosis related proteins-B-cell lymphoma-2 ( Bcl-2 ) and Bcl-2 associated x protein ( Bax ) were detected by Western blotting .Results ( 1 ) Both Live/dead and CCK-8 experiments indicated that the AuNSs/Col composite material with 0.1 mg/ml was nontoxicity to NRVMs and could further promote their proliferation .(2) Compared with the uninduced group , the early apoptosis rate of the Col group and the AuNSs /Col group after H2O2 induction was significantly increased , while the Bcl-2/Bax ratio was decreased , indicating that the oxidative stress damage model was established.After H2O2 induction, compared with the Col group , the early apoptosis rate of the AuNSs/Col group was decreased , but the Bcl-2/Bax ratio was increased .Conclusion AuNSs/Col composite material has protective effect on the oxidative damage of cardiomyocytes cultured in vitro.关键词
金纳米星/胶原/新生大鼠心室肌细胞/氧化性应激/过氧化氢/凋亡/纳米结构Key words
Au NanoStars(AuNSs)/collagen(Col)/newborn rat ventricular myocyte (NRVM)/oxidative stress/hydrogen peroxide/apoptosis/nanostructures分类
医药卫生引用本文复制引用
辛慧慧,李屹,白睿,魏玉杰,刘惠亮..金纳米星/胶原复合基质材料对新生大鼠心室肌细胞氧化应激损伤的影响[J].军事医学,2018,42(2):124-129,6.基金项目
国家自然科学基金资助项目(81700276) （81700276）
