江西农业学报2018，Vol.30Issue(5)：23-27,5.DOI:10.19386/j.cnki.jxnyxb.2018.05.05
白果越橘组织培养和快速繁殖技术研究
Study on Tissue Culture and Rapid Propagation of Vaccinium leucobotrys
摘要
Abstract
Vaccinium leucobotrys Nutt. is a sole white-fruit-type plant species in Vaccinium of Ericaceae, and it has an im?portant value in the breeding of huckleberry. The tissue culture techniques of V. leucobotrys were studied by using its fresh seeds, dry seeds or tender stems as explants. The results showed that the appropriate explants for the issue culture of V. leucobotrys were the fresh seeds in ripe fruit and the tender stems. The culture medium WPM+ 1.0 mg/L ZT was more conducive to the germination of seeds and the growth of axillary buds in tender stems. The culture medium WPM supplemented with 0.5 mg/L ZT was beneficial to the large amount of proliferation of V. leucobotrys. After seed germination, an oval and absinthe-green tissue formed on the base of the stem. The pH-value of culture medium had a great effect on the propagation of V. leucobotrys, and pH 5.4 was the best for its propagation. Thin humus soil is a kind of ideal cutting substrate, and it can replace moss and turfy soil to be used as the cutting substrate of huckleberry plantlets.关键词
白果越橘/新鲜种子/嫩茎段/pH值/腐殖土Key words
Vaccinium leucobotrys/Fresh seed/Tender stem/pH-value/Humus soil分类
农业科技引用本文复制引用
毕海林,杨正松,杨洪涛,黄杏娥,和志娇,木永青,和加卫..白果越橘组织培养和快速繁殖技术研究[J].江西农业学报,2018,30(5):23-27,5.基金项目
国家自然科学基金(31660550) （31660550）
科技惠民——云南省农科院农业科技创新及成果转化专项(2017RA01411). （2017RA01411）
