林业工程学报2018，Vol.3Issue(3)：73-80,8.DOI:10.13360/j.issn.2096-1359.2018.03.013
不同预处理对毛竹木质素抗氧化性的影响
Effects of different pretreatments on antioxidant activities of Moso bamboo lignin
摘要
Abstract
In this study, lignins in Moso bamboo and acid-pretreated bamboo were isolated according to the method de-veloped by Bjorkman, termed as MWLr and MWLa, respectively. Lignin in black liquor of pretreated bamboo by kraft pulping was precipitated by acid and termed as KL. Then, the obtained lignins ( MWLr, MWLa, and KL) were characterized by 2D heteronuclear single quantum correlation nuclear magnetic resonance ( 2D-HSQC NMR ) and quantitative 31 P NMR to analyze the effects of different pretreatments on the functional groups and types of lignin units in the resulting lignin. Meanwhile, the performance of above obtained lignins on the scavenging of superoxide anion free radical and 1,1-diphenyl-2-picryl-hydrazyl ( DPPH) radical were investigated. Results from the NMR analysis showed that the percentage of syringyl units in bamboo lignin increased from 50.6％ to 68.8％ and 72.2％ after it was pretreated by acid and kraft pulping, respectively. In addition, the pretreatments resulted in degradation of tricin and increase in the amounts of alphatic hydroxyl groups, phenolic hydroxyl groups, and carboxylic acid groups in obtained lignin samples. It showed that the capabilities of MWLr, MWLa, and KL to scavenge DPPH radical were up to 79.59％, 75.57％, and 77. 69％, respectively. For superoxide anion free radical, the highest scavenging rates were 81.15％, 47.27％, and 74.53％ that achieved by MWLr, MWLa, and KL, respectively. These results indicated that the antioxidant activity of the obtained lignin decreased after it was pretreated by acid or kraft pulping process. The probable reasons for this result might be due to the degradation of tricin in lignin and the increased percentage of sy-ringyl units in the lignin.关键词
毛竹木质素/预处理/核磁共振技术/官能团/抗氧化性Key words
Moso bamboo lignin/pretreatment/nuclear magnetic resonance/functional groups/antioxidant分类
化学化工引用本文复制引用
黄曹兴,房伶晏,赖晨欢,唐硕,梁辰,李鑫,勇强..不同预处理对毛竹木质素抗氧化性的影响[J].林业工程学报,2018,3(3):73-80,8.基金项目
国家自然科学基金面上项目(31570561) （31570561）
国家自然科学基金青年项目(31600463) （31600463）
广西清洁化制浆造纸与污染控制重点实验室开放基金( KF201725) （ KF201725）
江苏高校优势学科建设工程资助项目( PAPD). （ PAPD）
