日用化学工业2018，Vol.48Issue(3)：162-165,171,5.DOI:10.13218/j.cnki.csdc.2018.03.008
中华猕猴桃水提液清除羟自由基以及抑制酪氨酸酶活性的研究
Study on scavenging effect for hydroxyl radicals and inhibitory effect on tyrosinase in water soluble extracts from Chinese kiwifruit
摘要
Abstract
Scavenging capacity for hydroxyl radicals in water soluble extracts from Chinese kiwifruit was detected.The hydroxyl radicals were generated from Fenton reaction.The inhibitory action of water soluble extracts from Chinese kiwifruit on the activity of monophenolase and diphenolase contained in tyrosinase were studied by using L-tyrosinase and L-DOPA as the substrates.Results show that the water soluble extracts from Chinese kiwifruit can scavenge hydroxyl radicals effectively.The hydroxyl radical elimination ratio of the water soluble extracts achieves 95.57％ when the mass concentration is 1.0 g/L.The water soluble extracts from Chinese kiwifruit inhibit both monophenolase and diphenolase activities of tyrosinase effectively with IC50 at 0.28 and 0.35 g/L respectively.The study of kinetics show that the inhibitory mechanism of the water soluble extracts from Chinese kiwifruit on diphenolase is reversible,and the inhibitory type is the mixed-type inhibition.关键词
中华猕猴桃水提液/羟自由基/酪氨酸酶/动力学Key words
water soluble extracts from Chinese kiwifruit/hydroxyl radicals/tyrosinase/kinetics分类
化学化工引用本文复制引用
张青,徐勇威,刘薇,龚盛昭..中华猕猴桃水提液清除羟自由基以及抑制酪氨酸酶活性的研究[J].日用化学工业,2018,48(3):162-165,171,5.基金项目
2017年广州市科技计划项目产学研协同创新重大专项(201604016111) （201604016111）
