山东农业科学2018,Vol.50Issue(5):1-6,6.DOI:10.14083/j.issn.1001-4942.2018.05.001
棉花Bax inhibitor-1影响内质网胁迫介导的细胞死亡的研究
Overexpression of Cotton Bax inhibitor -1 Affects Cell DeathMediated by Endoplasmic Reticulum Stress in Arabidopsis
摘要
Abstract
Bax inhibitor -1 (BI -1) regulates cell death mediated by endoplasmic reticulum stress (ER stress), and plays important roles in plant stress tolerance .Up to now, the role of cotton BI -1 (GhBI -1) involved in stress tolerance and cell death remain largely unknown .In the present study, we characterized the expression patterns of GhBI -1 under ER stress conditions induced by tunicamycin ( TM).The results of real -time quantitative PCRs showed that the inducible expression pattern of GhBI -1 was tissue specific.The expression of GhBI -1 in roots is stronger compared with in leaves under ER stress conditions .The overex-pression of GhBI -1 increased the TM resistance of transgenic Arabidopsis and delayed cell death mediated by ER stress.The analysis of the expression of bZip60 involved in unfolded protein response showed that GhBI -1 activated the avtivity of bZip60 and improved cell tolerance to ER stress.关键词
Baxinhibitor-1/内质网胁迫/细胞死亡/棉花/拟南芥/衣霉素Key words
Bax inhibitor -1/Endoplasmic reticulum stress/Cell death/Cotton/Arabidopsis/Tunicamycin分类
农业科技引用本文复制引用
张景霞,霍雪寒,王芙蓉,张传云,张军..棉花Bax inhibitor-1影响内质网胁迫介导的细胞死亡的研究[J].山东农业科学,2018,50(5):1-6,6.基金项目
山东省农业科学院青年科研基金项目(2015YQN04) (2015YQN04)
国家自然科学基金项目(31501351) (31501351)