山东农业科学2018，Vol.50Issue(5)：168-172,5.DOI:10.14083/j.issn.1001-4942.2018.05.034
玉米Mutator转座子系统的应用及展望
Application and Prospect of Maize Mutator Transposon System
摘要
Abstract
The transposon tagging is a very effective molecular biology technology developed in recent years.It causes gene mutation through TEs insertion into the genomes of higher plants , and then the mutanted genes can be cloned by isolating the flanking sequence of TEs .This strategy is very useful in the study of the functional genomics of higher plants .The main systems of maize transposon are En/Spm, Ac/Ds and Mutator ones.Among them, the Mutator transposon was prone to inserting within or around the gene ; the positive mu-tation frequency was 10-5 ～10 -4 , which was nearly 30 times higher than that of the wild corn; and most of the mutations were recessive.The mutation can also be cloned by TAIL -PCR.Now by this method, some important genes have been cloned.In this paper, we mainly introduced the application of Mutator system, and discussed the research prospect of Mutator transposon system.关键词
玉米/Mutator转座子/应用/展望Key words
Maize/Mutator transposon/Application/Prospect分类
农业科技引用本文复制引用
高志勇,谢恒星,李吉锋,刘史力..玉米Mutator转座子系统的应用及展望[J].山东农业科学,2018,50(5):168-172,5.基金项目
陕西省教育厅2016年重点科学研究计划项目"渭南湿地植物的调查与开发利用研究"(16JS031) （16JS031）
陕西省2018年军民融合研究基金项目"渭南地区种子植物资源调查与应用研究"(18JMR50) （18JMR50）
