山东医药2018，Vol.58Issue(9)：13-16,4.DOI:10.3969/j.issn.1002-266X.2018.09.004
EPCR对人乳腺癌MDA-MB-231细胞增殖、迁移的影响及机制
Effects of EPCR on the proliferation and migration of human breast cancer cells MDA-MB-231
摘要
Abstract
Objective To explore the effects and mechanism of endothelial protein C receptor (EPCR) on the proliferation and migration of human breast cancer cells MDA-MB-231.Methods MDA-MB-231 cells were divided into the EPCR transfection group,unrelated sequence transfection group,and control group which were transfected with EPCR-siRNA,siRNA-NC,and empty plasmid,respectively.Cell-ELISA was used to detect the activation of PAR-1 in the membranes of MDA-MB-231.CCK-8 assay wasperformed to observe the proliferation of cells in each group.Transwell chamber was employed to observe the cell's migration.After treatment of anti-PAR-1 antibody,the proliferation and migration abilities of MDA-MB-23 1 cells were detected by CCK-8 and Transwell assay.Western blotting was used to determine the protein expression levels of Erk1/2 and AKT.Results Compared with the control group and unrelated sequence transfection group,the binding rate of un-cleaved activated PAR-1 antibody increased,and the proliferation and migration abilities significantly decreased in the transfection group (all P ＜ 0.05).Compared with the control group,after being treated with anti-PAR-1 antibody,the proliferation and migration of MDA-MB-231 cells significantly decreased (both P ＜0.05).After knockdown of EPCR or the PAR-1 antibody treatment,the expression of p-Akt (S473) and p-Akt (T308) significantly decreased in the MDA-MB-231 cells (both P ＜ 0.05),but the expression of p-Erk1/2 did not change significantly (P ＞0.05).Conclusion EPCR contributes to the proliferation and migration of MDA-MB-231 cells by activating AKT,and this effect of EPCR may be dependent on PAR-1.关键词
乳腺癌/内皮细胞蛋白C受体/蛋白酶活化受体1/细胞增殖/细胞迁移/蛋白激酶BKey words
breast carcinoma/endothelial protein C receptor/protease-activated receptor 1/cell proliferation/cell migration/protein kinase B分类
医药卫生引用本文复制引用
徐炎炎,卓倩,汤洋洋,王庆苓..EPCR对人乳腺癌MDA-MB-231细胞增殖、迁移的影响及机制[J].山东医药,2018,58(9):13-16,4.基金项目
国家自然科学青年基金资助项目(81101493). （81101493）
