山东医药2018，Vol.58Issue(13)：1-4,4.DOI:10.3969/j.issn.1002-266X.2018.13.001
宫颈癌组织中miRNA-125a、STAT3、 IL-10的表达变化及意义
Expression changes of miRNA-125a, STAT3, and IL-10 in cervical carcinoma tissues
摘要
Abstract
Objective To investigate the expression changes and significance of microRNA-125a (miR-125a),signal transduction and activator of transcription 3 (STAT3),and interleukin-10 (IL-10) in the cervical cancer tissues.Methods RT-PCR and qRT-PCR were used to detect the expression levels of miR-125a,signal transducer and activator of transcription 3 (STAT3),and IL-10 mRNA in 56 cases of cervical squamous cell carcinoma tissues (cervical cancer group) and 40 cases of normal cervical epithelial tissues (control group),and we analyzed the correlations between them in the cervical cancer group.The immunohistochemical Elivision two-step was used to detect the protein expression of STAT3 and IL-10 in the two groups.The relationship between the expression of miR-125a,STAT3 mRNA and clinicopathological features of cervical cancer patients was analyzed.Results Compared with the control group,the expression of miR-125a mRNA decreased in the cervical cancer group,and the mRNA expression of STAT3 and IL-10 increased (all P ＜0.05).The expression levels of STAT3 and IL-10 were positively correlated (r =0.457 8,P ＜ 0.05).Compared with the control group,the positive expression rate of STAT3 protein in the cervical cancer group increased (P ＜ 0.05).The expression of miR-125a was related to the differentiation of cervical cancer,lymph node metastasis,and clinical stage (all P ＜ 0.05).The expression of STAT3 was related to lymph node metastasis and clinical stage (both P ＜ 0.05).Conclusion The expression of miR-125a decreases,and the expression of STAT3 and IL-10 increases in the cervical cancer tissues.The three factors may interact and participate in the occurrence and development of cervical cancer.关键词
宫颈癌/微小RNA-125a/信号转导及活化因子3/白细胞介素10Key words
cervical carcinoma/miR-125a/signal transducer and activator of transcription 3/interleukin-10分类
医药卫生引用本文复制引用
李秀福,贾世峰,耿彤瑶,王新月,张琪,马冬,李鸥..宫颈癌组织中miRNA-125a、STAT3、 IL-10的表达变化及意义[J].山东医药,2018,58(13):1-4,4.基金项目
国家自然科学基金项目(81541149) （81541149）
河北省青年自然科学基金项目(H2016209095). （H2016209095）
