山东医药2018，Vol.58Issue(20)：17-20,4.DOI:10.3969/j.issn.1002-266X.2018.20.005
长链非编码RNA lucat1对胃癌细胞侵袭和迁移能力的影响及其机制
Effects of long non-coding RNA lucat1 on invasion and migration of gastric cancer cells
摘要
Abstract
Objective To investigate the effects of long non-coding RNA lucat1 on the invasion and migration abilities of gastric cancer ( GC) cells SGC-7901 and BGC-823 as well as its mechanism .Methods ① The mRNA expression of formyl peptide receptor 2 (FPR2) and lncRNA lucat1 in normal gastric mucosa cells (GSE-1) and GC cells (SGC-7901 and BGC-823) was detected by real-time fluorescent quantitative PCR;②SGC-7901and BGC-823 cells were divided into the observation group and control group , respectively .The cells in the observation group were transfected with lucat 1 shR-NA by lipofectamine 2000 for 48 h, and the cells in the control group were untreated .The mRNA expression of FPR2 in the SGC-7901and BGC-823 cells was examined by RT-PCR.③SGC-7901and BGC-823 cells were divided into groups A , B, and C.Cells in the group A were transfected with lucat 1 shRNA, cells in the group A were transfected with lucat 1 shRNA and over-expressed FPR2, and cells in the group C were not treated .The changes of invasive and metastasis abilities in SGC-7901 and BGC-823 cells at 48 h after transfection were examined by Scratch test and Transwell assay , respectively. Results ①The relative expression levels of lucat 1 in the SGC-7901, BGC-823, and GES-1 cells were 3.651 ±0.332, 3.023 ±0.331, and 1.000 ±0.098, respectively.The relative expression levels of FPR2 mRNA were 3.354 ±0.433, 3.425 ±0.354, and 1.000 ±0.277, respectively.The relative expression of lucat1 and FPR2 mRNA in SGC-7901 and BGC-823 cells was significantly lower than that in GES-1 cells (P<0.05).②The relative expression levels of FPR2 mR-NA of SGC-7901 cells in the observation group and control group were 1.00 ±0.05 and 0.48 ±0.04, respectively;the rel-ative expression levels of FPR2 mRNA in BGC-823 cells were 1.00 ±0.04 and 0.48 ±0.04, respectively, P<0.05.③The transmembrane cells in the groups A , B, and C were 143 ±7, 194 ±9, and 233 ±8; significant difference was found between every two group (all P<0.05), with the lowest in group A, and highest in group C.The migration distance of groups A, B, and C were (0.342 ±0.027), (0.534 ±0.021), and (0.652 ±0.029) mm, respectively, and signifi-cant difference was found between every two group (all P<0.05), with the lowest in group A, and highest in group C. Conclusion LncRNA lucat1 can promote the invasive and metastasis of GC cells , and it is associated with FDR2.关键词
胃癌/长链非编码RNA/lucat1/甲酰肽受体2/细胞侵袭/细胞迁移Key words
gastric carcinoma/long non-coding RNA/lucat1/formyl peptide receptor 2/cell invasion/cell metasta-sis分类
医药卫生引用本文复制引用
于贵林,林杰,赵国华,艾福禄,王越..长链非编码RNA lucat1对胃癌细胞侵袭和迁移能力的影响及其机制[J].山东医药,2018,58(20):17-20,4.基金项目
国家外国专家局科研基金项目(20172100003). （20172100003）
