生物加工过程2018，Vol.16Issue(3)：1-11,11.DOI:10.3969/j.issn.1672-3678.2018.03.001
ω-转氨酶不对称合成手性胺及非天然氨基酸的研究进展
ω-Transaminase for asymmetric synthesis of chiral amines and unnatural amino acids
摘要
Abstract
The asymmetric synthesis of chiral amines and unnatural amino acids using ω-transaminase as catalyst is one of the hot topics in bioprocess engineering. Because of its excellent stereo- and regio-selectivity,ω-transaminase has been widely used in the fields of medicine, pesticide and chemical industry.This review describes the basic structural characteristics of ω-transaminase and summarizes the recent research advances in the high-throughput screening assay development and protein engineering ofω-transaminase. The applications of ω-transaminase in asymmetric synthesis of chiral amines and the cascade-involved strategies for increasing chiral amine production are then discussed ( sitagliptin synthesis as an example was emphasized).Finally,the synthesis of unnatural amino acids by ω-transaminase was briefly introduced.关键词
ω-转氨酶/不对称合成/手性胺/非天然氨基酸/生物催化Key words
ω-transaminase/asymmetric synthesis/chiral amines/unnatural amino acids/biocatalysis分类
生物科学引用本文复制引用
程峰,相超,王亚军..ω-转氨酶不对称合成手性胺及非天然氨基酸的研究进展[J].生物加工过程,2018,16(3):1-11,11.基金项目
国家自然科学基金(31700693) （31700693）
浙江省自然科学基金(LQ17C050002) （LQ17C050002）
