生物加工过程2018，Vol.16Issue(3)：22-28,7.DOI:10.3969/j.issn.1672-3678.2018.03.003
多孔木霉色素的结构鉴定和合成基因簇分析
Identification of pigment and analysis of its related gene clusters from cyclosporine A-producing strain Tolypocladium inflatum
摘要
Abstract
To identify the chemical structure of pigment from Tolypocladium inflatum, we separated and analyzed the pigment by high pressure preparation liquid chromatography and spectroscopy analysis. Furthermore,we used online bioinformatic analysis software to predict the gene cluster and its function. The results showed that the pigment was an anthraquinone compound with chemical formula of C15H10O5, and had three phenoxyl radicals and two ketone groups. The related gene clusters of pigment contain 43 open reading frame ( ORFs ) and belong to polyketide synthase ( PKS ) type cluster. Our studies can provide a basis for further exploring the biosynthetic pathway of pigment.关键词
多孔木霉/色素/聚酮合酶/合成基因簇/环孢菌素A/生物医药Key words
Tolypocladium inflatum/pigment/polyketide synthase/biosynthetic gene cluster/cyclosporine A/biological medicine分类
生物科学引用本文复制引用
洪虹,岳雪,李大成,王炳全,董惠钧..多孔木霉色素的结构鉴定和合成基因簇分析[J].生物加工过程,2018,16(3):22-28,7.基金项目
山东省自然科学基金( ZR2015CL001) （ ZR2015CL001）
山东省抗体制药协同创新中心开放课题( CIC-AD1818) （ CIC-AD1818）
