生物加工过程2018，Vol.16Issue(3)：29-34,6.DOI:10.3969/j.issn.1672-3678.2018.03.004
控氧发酵对干酪乳杆菌合成苯乳酸的影响
Effect of oxygen on phenyl lactic acid production by Lactobacillus casei B3
摘要
Abstract
Phenyl lactic acid was produced by microbial fermentation and whole-cell biotransformation using Lactobacillus casei B3 under different oxygen control conditions, i. e. , the static culture, the agitation,and the aeration-agitation fermentations.Cell growth and glucose consumption were studied. The concentration of phenyl lactic acid was 41. 4％ higher in the agitation at the speed of 100 r/min, but 60. 3％ lower in the aeration-agitation at the speed of 100 r/min and aeration rate of 2 L/min than that in the static culture.Using phenyl pyruvic acid of 8 g/L as substrate,the molar conversion ratios for phenyl lactic acid with the cells from the static culture,the agitation,and the aeration-agitation processes as the whole-cell catalysts,were 67. 2％,62. 7％ and 35. 9％,respectively. The results indicated that the mass transfer of substrate and product was enhanced by the suitable agitation, while the activities of key conversion enzymes within the cells could be influenced by the enough oxygen supply regarding the aeration-agitation,and thus was negative to the whole-cell transformation.关键词
干酪乳杆菌/苯乳酸/控氧/发酵/生物转化Key words
Lactobacillus casei/phenyl lactic acid/oxygen control/fermentation/bioconversion分类
化学化工引用本文复制引用
韩朝飞,关今韬,富敏霞,张颂红,祝铃钰,贠军贤..控氧发酵对干酪乳杆菌合成苯乳酸的影响[J].生物加工过程,2018,16(3):29-34,6.基金项目
国家自然科学基金(21576240) （21576240）
浙江省自然科学基金(LZ14B060001、LY16B060011) （LZ14B060001、LY16B060011）
