生物加工过程2018，Vol.16Issue(3)：47-52,6.DOI:10.3969/j.issn.1672-3678.2018.03.007
乌梅有机酸的提取工艺及其抑菌活性
Extraction and antibacterial activity of Fructus mume organic acids
摘要
Abstract
We optimized the extraction of organic acid from Fructus mume to study its antibacterial activity. Firstly, effects of different extraction methods, solvent concentration, extraction time, ultrasonic frequency and ratio between solid to liquid on the yield of organic acids were studied by single-factor experiments. The optimum extraction process was determined by orthogonal experiment. The optimum extraction conditions were ultrasonic-assisted extraction, 95％ ethanol ( V/V ) , solid-liquid ratio 1: 25 (g/mL),extraction time 60 min and extraction frequency 100 kHz.The yield of organic acids from Fructus mume reached 12. 17％.Fructus mume extract inhibited Bacillus subtilis,Staphylococcus aureus,Escherichia coli and Micrococcus tetragenus, the minimum bacteriostat mass concentrations were 15. 625, 15. 625, 31. 25 and 31. 25 mg/mL,respectively.Ultrasonic-assisted extraction of organic acids from Fructus mume was effective and the extract inhibited several bacteria.关键词
乌梅/有机酸/提取工艺/抑菌活性Key words
Fructus mume/organic acid/extraction process/antibacterial activity分类
医药卫生引用本文复制引用
张丹丹,姜修婷..乌梅有机酸的提取工艺及其抑菌活性[J].生物加工过程,2018,16(3):47-52,6.基金项目
山东省高等学校科技计划( J15LM62) （ J15LM62）
