山西农业大学学报（自然科学版）2018，Vol.38Issue(6)：29-34,6.DOI:10.13842/j.cnki.issn1671-8151.201801028
3种早春植物叶片蛋白质SDS-PAGE分析
Study on protein extraction method of three early spring plant by SDS-PAGE
陈虹 1李涛 1白晋华 1郭红彦1
作者信息
- 1. 山西农业大学 林学院 ,山西 太谷 030801
- 折叠
摘要
Abstract
[Objective] The suitable method of leaf protein extraction for early spring plant was screened out from four different ways .The variation of protein content and composition at different leaf stagesin three rosaceous species were determined and analyzed . .The results provided the theoretical basis for the corresponding proteomics research in the later stage .[Methods] Four kinds of protein extraction methods ,known as phenolic extraction ,modified phenolic method ,modified Tris-HCl method ,and TCA-acetone extraction method were employed .The protein content and com-position of three early spring plant leaves were determined by (G-250) staining and SDS-PAGE .[Results] Study indi-cated that TCA-acetone extraction method produced better protein profile and higher quality of gel electrophoresis pat -tern compared to other extraction methods .During April to early June ,the protein content in the leaves at five differ-ent growth stages showed "first increase ,then decrease" trend" ,and in different growth stages Sorbaria kirilowii leaf protein content was significantly lower than Cerasus serrulata ,lannesiana ,and Malus micromalus .SDS-PAGE elec-trophoresis showed that three early spring plant leaves were rich in various protein components .From April to May , the protein expression of Cerasus serrulata lannesiana and Malus micromalus leaves were relatively higher and the bands were clearer .The protein bands of Sorbaria kirilowii leaves and the protein types that were changed during flow -ering stage from June to July were relatively fewer than other species .[Conclusion] The TCA-acetone extraction meth-od was the optimal protein extraction method for three kinds of rosaceous plants .In early spring ,protein content of ro-saceous plants was closely affected by the flowering time ,and the protein content before flowering was constantly in -creased and reached the peak at full-blooming stage .The protein content of early flowering species ,such as Cerasus serru-lata lannesiana and Malus micromalus ,was greater than that of late flowering species ,such as Sorbaria kirilowii .关键词
日本晚樱/西府海棠/华北珍珠梅/蛋白质/SDS-PAGEKey words
Cerasus serrulata lannesiana/Malus micromalus/Sorbaria kirilowii/Protein/SDS-PAGE分类
农业科技引用本文复制引用
陈虹,李涛,白晋华,郭红彦..3种早春植物叶片蛋白质SDS-PAGE分析[J].山西农业大学学报（自然科学版）,2018,38(6):29-34,6.
