山西农业大学学报（自然科学版）2018，Vol.38Issue(6)：71-76,6.DOI:10.13842/j.cnki.issn1671-8151.201803021
戊二醛交联的传感器检测布鲁氏菌抗体的研究
Developing glutaraldehyde cross-linking based immunosensors for Brucella melitensis antibody detection
摘要
Abstract
[Objective] A novel method was established to detect minimum low concentrations of Brucella melitensis an-tibody for early diagnosis of brucellosis .[Methods]Both original and ultrasonic broken Brucella melitensis antigens were crosslinked and entrapped on the pretreated screen-printed gold electrode surface by glutaraldehyde .Then the im-mune responses of antigens were characterized by cyclic voltammetry ,different wave voltammetry ,and square pulse voltammetry ,and a label-free amperometic immunosensor was constructed .[Results] A linear function between peak current and the logarithm concentration of Brucella melitensis antibody was found in the range of 1 × 10 - 5 ～ 1 × 10 IU·mL - 1 .The immunosensor system tested by square wave voltammetry exhibited the highest sensitivity ,and the lowest detection limit at 1.2065 × 10 - 9 IU·mL - 1 .[Conclusion] The results showed that the immunosensor pretreated with glutaraldehyde cross-linking could effectively detect Brucella melitensis antibody at low concentration .关键词
戊二醛/丝网印刷金电极/免疫传感器/布鲁氏菌抗体/超声破碎抗原/伏安法Key words
Glutaraldehyde/Screen-printed gold electrode/Immunosensor/Brucella melitensis antibody/Ultrasonic fragmentation antigen/Voltammetry分类
信息技术与安全科学引用本文复制引用
杨威,韩小平,张志勇,左月明..戊二醛交联的传感器检测布鲁氏菌抗体的研究[J].山西农业大学学报（自然科学版）,2018,38(6):71-76,6.基金项目
国家自然科学基金(30871445) （30871445）
山西省回国留学人员资助项目(2013-061) （2013-061）
山西农业大学青年科技创新基金项目(201503) （201503）
