陕西医学杂志2018，Vol.47Issue(6)：683-685,3.DOI:10.3969/j.issn.1000-7377.2018.06.001
miR-34a通过下调SIRT1抑制乳腺癌干细胞机制研究
Effect of miR-34a on downgrading SIRT1 in breast cancer stem cells
摘要
Abstract
Objective :To study the mechanism of miR-34a inhibiting breast cancer stem cells by down regula-ting SIRT1 .Methods :The mimics or inhibitors of miR-34a were synthesized by chemical synthesis method ,and the MCF-7 cells were infected with qRT-PCR ,and the expression of miR-34a gene was confirmed by the method of . Lentiviral vector was used as a vector to express the miR-34a gene ,and the cell lines with stable infection were screened out .Design of SIRI1 gene shRNA ,used to interfere with the expression of SIRT 1 in breast cancer MCF-7 cells .Detection of mRNA and protein expression in SIRT 1 by qRT-PCR and Western blotting .Results :No more than the expression level of mimics cells transfected by miR-34a transfected cells miR-34a gene transfected cells ,no less than the expression level of inhibitors cells transfected with miR-34a miR-34a gene ,the difference was statisti-cally significant (P< 0 .05) .LV-miR-34a infected cells can up regulate the expression of miR-34a ,compared with non transfected cells ,the difference was statistically significant (P< 0 .05) .The expression of mRNA and protein in SIRT1 and shRNA-SIRT1-4 group decreased in the shRNA-SIRT1-3 group . Overexpression of miR-34a had no effect on the expression of mRNA in SIRT 1 .miR-34a mimics can inhibit the expression of SIRT 1 protein ,the ex-pression of miR-34a inhibitors is upregulated SIRT 1 protein ,compared with non transfected breast cancer cells of MCF-7 ,the difference was statistically significant (P< 0 .05) .Conclusion :The expression of miR-34a can be regula-ted by the miR-34a mimics and inhibitors ,which can change the expression of SIRT1 protein .关键词
@miR-34a/@SIRT1/乳腺肿瘤/病理学/肿瘤干细胞/抑制因子,免疫Key words
@ MiR-34a/@ SIRT1/Breast Neoplasms /pathology/Neoplasms stem cells/Suppressor fac-tors,immunologic分类
医药卫生引用本文复制引用
李晓锋,汪园园,刘希..miR-34a通过下调SIRT1抑制乳腺癌干细胞机制研究[J].陕西医学杂志,2018,47(6):683-685,3.基金项目
陕西省国际科技合作与交流计划项目(2016KW-009) （2016KW-009）
