厦门大学学报（自然科学版）2018，Vol.57Issue(3)：346-353,8.DOI:10.6043/j.issn.0438-0479.201707010
环境因子对微藻胞外多聚物主要组分的影响
The Effect of Environmental Factors on the Principal Conponents of Microalgal Extracellular Polymeric Substances
摘要
Abstract
Extracellular polymeric substances (EPS)have an important effect on cell growth,flocculation,biofilm formation,etc.In this paper,one strain of Botryococcus ,one strain of Scenedesmus ,and two strains of Chlorella were selected to investigate the poly-saccharide and protein in EPS when the nitrogen content,pH,temperature and the culture mode changed.The results showed that the nitrogen-deficient growth environment caused a reduction of up to 56.0％ in protein.Polysaccharides accounted for 58.08％ to 80.78％ of EPS,while proteins accounted for 5.73％ to 13.45％.There were more polysaccharides and proteins in Chlorella strains than in other species.As elaborated in the representative Botryococcus strain,when the pH value was 11,the polysaccharide and pro-tein contents (per gram of cell weight)in EPS approximately increased by 0.135 g and 0.018 g,respectively,compared with the data at pH 7.The content of polysaccharide also increased from 0.014 g to 0.600 g as the temperature decreased.When the culture mode changed,the polysaccharide content in EPS of the heterotrophic cultivation was higher than that of the autotrophic cultivation,while the protein content showed the opposite trend.关键词
微藻/胞外多聚物/多糖/蛋白质Key words
microalgae/extracellular polymeric substances(EPS)/polysaccharide/protein分类
生物科学引用本文复制引用
吴琪璐,崔文倩,沈亮,卢英华..环境因子对微藻胞外多聚物主要组分的影响[J].厦门大学学报（自然科学版）,2018,57(3):346-353,8.基金项目
国家自然科学基金(21736009) （21736009）
福建省高校青年自然基金重点项目(JZ160401) （JZ160401）
厦门市科技计划项目(3502Z20173018) （3502Z20173018）
