厦门大学学报（自然科学版）2018，Vol.57Issue(3)：432-437,6.DOI:10.6043/j.issn.0438-0479.201801006
康复新液对转化生长因子-β1诱导人胚肺成纤维细胞MRC-5纤维化模型的干预作用
Effect of Kangfuxin Liquid in Alleviating Human Fetal Lung Fibroblast MRC-5 Cell Fibrosis Model Induced by Transforming Growth Factor-β1
摘要
Abstract
To investigate the effect of Kangfuxin liquid on the pulmonary fibrosis cell model,the pulmonary fibrosis cell model was established by treating human fetal lung fibroblast MRC-5 cell with transforming growth factor-β1(TGF-β1),and the toxic effect of Kangfuxin liquid on MRC-5 cell was detected by cell-counting kit-8(CCK-8)assay.Then,Kangfuxin liquid diluted by 80 times was implemented on MRC-5 cell model induced by TGF-β1,and the mRNA and protein expression levels of fibrosis indexes were detected by qRT-PCR and Western blotting.The results showed that the pulmonary fibrosis cell model was established by treating human fe-tal lung fibroblast MRC-5 cell with TGF-β1 at the dose of 10 ng/mL.Kangfuxin liquid could significantly alleviate pulmonary fibrosis degree and efficiently down-regulate expression levels of α-SMA,LN,FN,CTGF,Collagen Ⅰ and Collagen Ⅲ in the pulmonary fi-brosis cell model,with the comparable effect with Dexamethasone.Thus Kangfuxin liquid could significantly alleviate pulmonary fi-brosis degree and might be a potential anti-fibrosis drug.关键词
康复新液/肺纤维化/转化生长因子-β1/MRC-5细胞Key words
Kangfuxin liquid/pulmonary fibrosis/transforming growth factor-β1/MRC-5 cell分类
医药卫生引用本文复制引用
徐秋颖,刘伟伟,王宝家,马秀英,高永翔..康复新液对转化生长因子-β1诱导人胚肺成纤维细胞MRC-5纤维化模型的干预作用[J].厦门大学学报（自然科学版）,2018,57(3):432-437,6.基金项目
成都市科技惠民项目(2015-HM01-00466-SF) （2015-HM01-00466-SF）
