烟草科技2018，Vol.51Issue(5)：1-7,7.
不同培养条件下纳米碳溶胶对烟草细胞生长的影响
Effects of nano-carbon sol on tobacco cell growth under different culture conditions
摘要
Abstract
In order to investigate any promoting effect and associated physiology by nano-carbon sol, culture experiments were carried out under solid and liquid culture conditions. The effects of nano-carbon sol application on the expression of related genes including water channel protein gene and cell division gene, biomass, nutrient accumulation and suspension cell growth characteristics of tobacco BY-2 cells were studied. The results showed that the addition of nano-carbon sol enhanced the growth of tobacco callus and increased the accumulation of N, P and K nutrients. In all the treatments, 50 mg/L concentration of nano-carbon sol had the largest increase rates of nutrient accumulation in callus, namely 41.3％, 27.6％ and 47.0％, respectively. At the same time, nano-carbon sol significantly increased the expression levels of aquaporin gene NtPIP1 and cyclin gene CycB at early culture stage, which played an important role for promoting callus growth. In suspension culture experiments, nano-carbon sol presented a good biocompatibility at the early and middle culture stages without significantly affecting the density, packed cell volume and mortality of the suspension cells. At the late culture stage, nano-carbon sol had an inhibitory effect at the concentration of 10 mg/L, which decreased the cell density and cell viability, which is likely to be associated with the aging of cultured cells. In conclusion, the effects of nano-carbon sol on the growth of tobacco cells were influenced by the culture conditions and application concentration.关键词
烟草/纳米碳溶胶/愈伤组织/悬浮细胞/生物量Key words
Tobacco/Nano-carbon sol/Callus/Suspension cell/Biomass分类
农业科技引用本文复制引用
赵振杰,梁太波,王宝林,陈千思,胡利伟,张艳玲,孟祥宇,尹启生..不同培养条件下纳米碳溶胶对烟草细胞生长的影响[J].烟草科技,2018,51(5):1-7,7.基金项目
中国烟草总公司郑州烟草研究院科技项目"纳米碳对烟草促生作用的机理研究"(112013CZ0580) （112013CZ0580）
中国烟草总公司郑州烟草研究院院长基金项目"基于非损伤微测技术的烟草根系钾离子流速研究"(112017CA0090). （112017CA0090）
