双调蛋白抗体对双眼诱导型近视豚鼠眼部的影响
Effects of amphiregulin antibody on the eyes of guinea pigs with binocular lens-induced myopic
摘要
Abstract
Objective To investigate the effects of amphiregulin antibody on the axial length,diopter and posterior sclera thickness in eyes of bilateral lens-induced myopic guinea pigs.Methods A total of 60 healthy three-colored short-hair guinea pigs were randomly divided into 5 groups:myopic model group,low dose group,medium dose group,high dose group and normal control group,12 rats in each group,and both eyes of the guinea pigs in the former four groups were induced by-10 D lens,while the normal group did not make any treatment.After wearing of goggles for 2 weeks,the right eyes of guinea pigs were intravitreally administrated with 5 μg,10 μg,20 μg anphiregulin antibody for the low,medium and high dose group,respectively.At the same time,the left eyes of these groups were given the same dose of Ringer' s buffer (buffer group).Continuous wearing of the lenses before and after injection of antibody was allowed.Diopter and axial length of guinea pigs were measured before wearing of the lenses,2 weeks after wearing lenses and 5 weeks after the experiment (after 3-times injections of antibody).Moreover,the thicknesses of retina,choroid and sclera in the posterior pole were detected by PAS staining.Finally,the expression of amphiregulin and EGFR mRNA and protein was detected by real time quantitative PCR and immunofluorescence.Results After 2 weeks of lens induction,the axial length of the myopic model group increased,but the diopter decreased when compared with the normal control group,and the differences were statistically significant (both P ＜ 0.05).Compared with the buffer group,after intravitreal injection of amphiregulin antibody,the axial length in the low,medium,and high dose groups decreased,while the diopter increased,and the scleral thickness at the posterior pole increased significantly in a dose-dependent manner,with statistically significant differences (all P ＜ 0.05).However,there was no significant change in retinal thickness at the posterior pole of all groups.Real-time quantitative PCR and immunofluorescence showed the expression of amphiregulin mRNA and protein in the retina was upregulated the myopic model group and buffer group,but downregulated in the high,medium,and low dose groups.Furthermore,when compared with the buffer group,the expression level of epidermal growth factor receptor in the retina of the low dose group was decreased (t =2.606,P =0.022),but there was no significant difference in the other groups.Conclusion After injection of amphiregulin antibody into the eyes of bilateral lens-induced myopic guinea pigs,the diopter increases,but the axial length is significantly shortened,and the posterior sclera is thickened,which may involve a decrease in the expression of amphiregulin.关键词
双调蛋白/双眼诱导型近视/眼轴长度/屈光度/后极部巩膜厚度Key words
Amphiregulin/Binocular lens-induced myopia/axial length/diopter/posterior scleral thickness分类
医药卫生引用本文复制引用
李少玉,宋惠欣,蒋文君,吴建峰,毕宏生..双调蛋白抗体对双眼诱导型近视豚鼠眼部的影响[J].眼科新进展,2018,38(6):510-514,5.基金项目
国家科技支撑计划项目(编号:2015BAI04B04) （编号:2015BAI04B04）
国家自然科学基金项目(编号:81603421) （编号:81603421）
山东省自然科学基金(编号:ZR2014HQ024) （编号:ZR2014HQ024）
山东省科技发展计划和重点研发计划(编号:2014GGH219004、2015GSF118043、2016GGH3119) （编号:2014GGH219004、2015GSF118043、2016GGH3119）
山东省医药卫生科技发展计划(编号:2015WS0141) （编号:2015WS0141）
山东省中医药科技发展计划(编号:2015-148)National Science and Technology Support Program (No:2015BAI04B04) （编号:2015-148）
National Natural Science Foundation of China (No:81603421) （No:81603421）
Natural Science Foundation of Shandong Province(No:ZR2014HQ024) （No:ZR2014HQ024）
Shandong Province Science and Technology Development Plan and Key R & D Plan (No:2014GGH219004,2015GSF118043,2016GGH3119) （No:2014GGH219004,2015GSF118043,2016GGH3119）
Shandong Province Medical and Health Technology Development Plan (No:2015WS0141) （No:2015WS0141）
Shandong Science and Technology Development Plan of Traditional Chinese Medicine (No:2015-148) （No:2015-148）
