中风与神经疾病杂志2018，Vol.35Issue(5)：388-394,7.
Bcl-2、Bad在大鼠癫痫持续状态中的表达及rHuEpo干预的分子机制
Effect of recombinant human erythropoietin on the expression of Bcl-2、Bad in hippocampus of status epileptic rats
摘要
Abstract
Objective To observe the hippocampal neuron damage of status epilepticus ( SE) SD rats kindled by Pentylenetetrazol ( PTZ) ,and the changes of neurons apoptosis and the expression changes of Bad 、Bcl-2 post the application of recombinant human erythropoietin (rHuEpo) and phosphatidyl inositol 3-kinase(PI3K) inhibitor LY294002,then discuss the possible mechanisms of rHuEpo furtherly .Methods The SE rats kindled by the PTZ were randomly divided into nor-mal control group (normal saline,NS),model group (PTZ+NS),rHuEpo treated group (PTZ+rHuEpo),LY294002 trea-ted group(PTZ+LY294002+rHuEpo),LY294002 control group (rHuEpo+PTZ+DMSO).The apoptosis of hippocampal neurons were detected by TUNEL method;The positive expression neurons of phosphorylation protein kinase B ( p-Akt)、Bcl-2 and Bad were detected by immunohistochemistry SP method;The expression of Bcl-2 mRNA、Bad mRNA in hippocampal neurons of each group of rats were detected through reverse transcription polymerase chain reaction ( RT-PCR) method;The expression of p-Akt and Bcl-2、Bad protein in hippocampal neurons of each group of rats were detected through Western blot method.Results rHuEpo can promote the expression of p-Akt、 Bcl-2 protein and Bcl-2 mRNA while down-regulate the expression of Bad protein and Bad mRNA and play a neuroprotective role .The expression of Bad protein and Bad mRNA in hippocampus increased significantly while the p-Akt、Bcl-2、Bcl-2 mRNA decreased significantly compared with that in rHuEpo treated group post the application of PI 3K inhibitor LY294002,which decreased the protective effects of rHuEpo and the difference was statistical significant .Conclusion From two aspects of positive and negative hints rHuEpo anti-apoptotic effect may through the impact of PI 3K/Akt signal pathway ,thus mediate the expression level of Bcl-2 and Bad pro-tein in the mitochondrial pathway of apoptosis ,finally achieve the neuroprotective effect against injury of status epilepticus apoptosis.关键词
重组人红细胞生成素/癫痫持续状态/磷脂酰肌醇3激酶/蛋白激酶B/B淋巴细胞瘤2/Bcl-xl/Bcl-2相关死亡启动因子Key words
Recombinant human erythropoietin (rHuEpo)/Status epilepticus(SE)/Phosphorylated protein ki-nase B(PKB/p-Akt)/Bcl-2/Bad分类
临床医学引用本文复制引用
于江华,冯晓红,尹阔场,王维平,史志勤,史诺菲,苏旭东,周毅,王珊,贾丽景,赵博,朱梦楚..Bcl-2、Bad在大鼠癫痫持续状态中的表达及rHuEpo干预的分子机制[J].中风与神经疾病杂志,2018,35(5):388-394,7.基金项目
国家自然科学基金面上项目(81671292) （81671292）
河北省卫计委重点课题(20160658) （20160658）
