中风与神经疾病杂志2018，Vol.35Issue(5)：409-414,6.
NQO1在tBHQ干预急性CO中毒后迟发性脑病大鼠海马区的表达及作用
The expression and effects of NQO1 in hippocampus of rats with tBHQ treatment on delayed encephalopathy after acute carbon monoxide poisoning
摘要
Abstract
Objective To investigate the expression of NQO 1 after tert-butylhydroquinone ( tBHQ) intervented those hippocampus of rats with delayed encephalopathy after acute carton monoxide poisoning ( DEACMP ) and its functions . Methods From 180 rats random drawing 60 rats as air control group (AC group), 60 rats as carbon monoxide poisoning group (CO group),60 rats as carbon monoxide poisoning +tBHQ group (TC group),and then divided into 6 subgroups after exposure,1 d、3 d、7 d、14 d、21 dand28 d according to the intraperitoneal injection of different solvents ,and the car-boxyhemoglobin ( HbCO) concentration in arterial blood was dynamic monitored .The Morris water maze was used to ob-served the average escape latency across the platform ,the platform quadrant swim time frequency to record the changes of learning ability and memory of rats ,Tunnel method was used to test the pyramidal cell apoptosis in the hippocampal CA 1 ar-ea.The expression of NQO1 was tested by immunohistochemical method and Western Blot method .Results DEACMP model was successfully established in 120 rats;The concentration of HbCO in rats continue to maintain more than 50％a-bove 16 hours throughout the course of exposure .The poisoned rats was found began to decline the ability of learning and memory in water maze test in the 14th day.Those changes in TC group was statistically significant (P<0.05) compared with CO group.The apoptosis index (AI) started to increase from first day and achieved it 's peak at the 7th day (7 d:TC group:23.041.89,CO group:25.522.11)in TC and CO group,then began to decrease slowly .But in the 1stday,3rdday and 7thday after exposured,the AI was significantly decreased(P<0.05),in the 21thday,28thday after exposured,the AI was significantly increased(P<0.05) compared with CO group.In TC and CO group the expression of NQO1 protein star-ted to increase from 1st day,reached its peak at 3rd day (TC group:9.560.83,CO group:8.080.58)and expressed signif-icantly higher than AC group at each time point (P<0.05).The difference between each peak point and that at other time points in the same group was statistically significant (P<0.05).Conclusion The NQO1 has a positively correlated with cell apoptosis ,and it rapidly increased in first 3 d to against oxidative stress injury after exposure play a protective effect . But it continuously has been excessive expressed driving the apoptosis of hippocampus pyramidal cells and may be an accel -eration of DEACMP .关键词
CO中毒/大鼠/海马/醌氧化还原酶1/叔丁基对苯二酚Key words
Carbon monoxide poisoning/Rats/Hippocampus/NQO1/tBHQ分类
临床医学引用本文复制引用
吴沙,吕霞,何林,张亦雯,张益梅,李经伦..NQO1在tBHQ干预急性CO中毒后迟发性脑病大鼠海马区的表达及作用[J].中风与神经疾病杂志,2018,35(5):409-414,6.基金项目
四川省卫生厅科研基金项目No.09095 （）
