中国动物检疫2018，Vol.35Issue(6)：5-7,3.DOI:10.3969/j.issn.1005-944X.2018.06.002
陕西省靖边县奶牛布鲁氏菌病血清学调查
Serological Investigation on Cow Brucellosis in Jingbian County of Shaanxi Province
盛丽芬 1张利 1董平 1武艳 1王宇慧 1白崇生2
作者信息
- 1. 靖边县动物疫病预防控制中心,陕西靖边 718500
- 2. 榆林市动物疫病预防控制中心,陕西榆林 719000
- 折叠
摘要
Abstract
In order to recognize the prevalence status of cow Brucellosis in Jingbian County of Shaanxi Province,a serological investigation was carried out during July to August of 2017. A total of 1227 serum samples were collected from 98 dairy farms and households and detected by three serological methods of the rose bengal plate agglutination test(RBPT),tube agglutination test(SAT)and competitive enzyme-linked immunosorbent assay(C-ELISA). The results showed that the group positive rate of Brucellosis was 13.27％,the individual positive rate was 3.50％. Among them,the individual positive rate of backyard households was 7.52％,which was higher than the standard of control area in China. In order to discuss the consistency of the above three detection methods,the results of the three detection methods were compared and analyzed. Results showed that the agreement rate of the three methods of RBPT,SAT and C-ELISA was higher than 99％,and theKappavalue was larger than 0.90,indicating a good consistency. Therefore, during the process of surveillance and purification of cow Brucellosis,combined application of the three detection methods could effectively reduce the probability of false positives and negatives,accurately identify the cow infected with wild strains,so as to reduce the mistaken and omissive slaughtering.关键词
布鲁氏菌病/奶牛/血清学调查/虎红平板凝集试验/试管凝集试验/竞争酶联免疫吸附试验Key words
Brucellosis/cow/serological investigation/RBPT/SAT/C-ELISA分类
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盛丽芬,张利,董平,武艳,王宇慧,白崇生..陕西省靖边县奶牛布鲁氏菌病血清学调查[J].中国动物检疫,2018,35(6):5-7,3.
