中国动物检疫2018，Vol.35Issue(6)：89-93,5.DOI:10.3969/j.issn.1005-944X.2018.06.024
河北省猪繁殖与呼吸综合征病毒遗传变异分析
Genetic Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus in Hebei Province
董李学 1袁万哲 2左玉柱 2王建昌 3王金凤3
作者信息
- 1. 唐山市畜牧水产品质量监测中心,河北唐山 063000
- 2. 河北农业大学动物医学院,河北保定 071001
- 3. 河北出入境检验检疫局检验检疫技术中心,河北石家庄 050051
- 折叠
摘要
Abstract
In order to recognize the prevalence status of Porcine reproductive and respiratory syndrome virus(PRRSV) and its genetic variation trend in pigs in Hebei Province,the 18 pig farms that were attacked by PRRSV in 10 areas were investigated. Applying the RT-PCR,a total of 23 virus strains with partial loss ofNSP2 gene and complete loss of ORF5 gene were identified. The sequence analysis results showed that all the 23 strains exhibited a 30 discrete amino acid deletion,confirming the prevalence situation of PRRSV variant strain of America type in Hebei Province. According to the phylogenetic tree ofORF5 gene drew by reference to the 23 isolates and the gene sequences published in and outside China,there were 20 isolates belonging to the subgroup that included JXA1 strain,and the other 3 isolates belonged to the subgroup that included DADC30 strain. With the largest variability,the latter 3 isolates would be the main objects of future clinical research. At last,the comparison results of amino acid showed there was much variation in the protein encoded byORF5 gene in the prevalent PRRSV strain in Hebei Province,therefore more attention should be raised in the process of PRRS future clinical diagnosis and prevention and control by vaccine.关键词
猪繁殖与呼吸综合征病毒/美洲型/基因变异分析/NSP2基因/ORF5基因Key words
PRRSV/America type/genetic variation analysis/NSP2 gene/ORF5 gene分类
农业科技引用本文复制引用
董李学,袁万哲,左玉柱,王建昌,王金凤..河北省猪繁殖与呼吸综合征病毒遗传变异分析[J].中国动物检疫,2018,35(6):89-93,5.
