中国动物检疫2018，Vol.35Issue(6)：94-97,4.DOI:10.3969/j.issn.1005-944X.2018.06.025
表达猪流行性腹泻病毒保护性抗原重组乳酸杆菌的构建及鉴定
Construction and Identification of Recombinant Lactobacillus casei Expressing Protective Antigens of Porcine Epidemic Diarrhea Virus
摘要
Abstract
In order to develop a safe and effective oral vaccine for Porcine epidemic diarrhea(PED),the core neutralizing epitope(COE)gene of PEDV was cloned and inserted into the shuttle expression vector. Then the vector was electric-transformed into the genetically engineered strain ofLactobacilluscasei. After bacteria culture and protein induction,a stripe of about 27 KD was detected by western blot. As a result,the develop of recombinantLactobacillus laid foundation for further study of animal immunological experiments in vivo and novel PED mucosal vaccine.关键词
猪流行性腹泻病毒/重组乳酸杆菌/COE基因/黏膜免疫/口服疫苗Key words
Porcine epidemic diarrhea virus(PEDV)/recombinantLactobacillus/core neutralizing epitope(COE) gene/mucosal immunization/oral vaccine分类
农业科技引用本文复制引用
孙玉章,许运斌,王宏华,孙明军,蒋贻海..表达猪流行性腹泻病毒保护性抗原重组乳酸杆菌的构建及鉴定[J].中国动物检疫,2018,35(6):94-97,4.基金项目
国家重点研发计划专项(2016YFD0501308) （2016YFD0501308）
