中国动物检疫2018，Vol.35Issue(6)：101-107,7.DOI:10.3969/j.issn.1005-944X.2018.06.027
鳗弧菌免疫诱导牙鲆IL-1β、IFN-γ和TNF-α基因表达变化
Variations of IL-1β,IFN-γ and TNF-α Gene Expression in Paralichthys olivaceus Vaccinated with Inactivated Vibrio anguillarum
摘要
Abstract
Mucosal immunity is an important component of immune system in fish,and the polymeric immunoglobulin receptor(pIgR)plays critical role in mediating the transportation and secretion of mucosal immunoglobulin(Igs), while the cytokineIL-1β,IFN-γ andTNF-α can regulate the expression of pIgR. In this study,by quantitative real-time polymerase chain reaction(qRT-PCR),the expression changes ofIL-1β,IFN-γ andTNF-α were detected in the tissues ofParalichthys olivaceus after immunization. The results revealed that the expressions of the three genes were all up-regulated by both intraperitoneal injection and immersion immunization. The up-regulated expression of the three genes first increased within96 hours and then decreased to the level of control group,and the expression levels ofIL-1β and IFN-γ were higher thanTNF-α,which were 10 to 60 folds higher than those in the control group,while the expression level ofTNF-α was just 2 to 4 folds higher than that in the control group. Some differences were observed in expressions of the three genes when the immunization methods were different. Immersion immunization had a greater influence on the expression of genes in the mucosal immune tissues(skin and sputum),the up-regulation time was short and the peak value was high. On the other hand,intraperitoneal injection had lager effects on the expression of genes in systemic immune tissues(spleen,head kidney and liver). In spleen and head kidney,the expression changes ofIL-1β andIFN-γ caused by injection immunization were 50 to 60 and 30 folds higher than the level in the control group,respectively,while the expression changes caused by immersion immunization were just 15 and 10 folds higher. In foregut,midgut and hindgut,similar up-regulation of IL-1β,IFN-γ andTNF-α expression was observed by the two immunization methods,or a slightly lower expression was found in the group of immersion immunization. As a conclusion,these results would provide further information for studying the regulation of pIgR expression by the cytokinesIL-1β,IFN-γ andTNF-α.关键词
牙鲆/鳗弧菌/白介素1β/干扰素γ/肿瘤坏死因子α/多聚免疫球蛋白受体Key words
Flounder(Paralichthys olivaceus)/Vibrio anguillarum/IL-1β/IFN-γ/TNF-α/pIgR分类
农业科技引用本文复制引用
钱晓宇,王占飞,绳秀珍,唐小千,邢婧,战文斌..鳗弧菌免疫诱导牙鲆IL-1β、IFN-γ和TNF-α基因表达变化[J].中国动物检疫,2018,35(6):101-107,7.基金项目
国家自然科学基金项目(31472295,31730101,31672685) （31472295,31730101,31672685）
泰山学者特聘专家项目 （）
