局解手术学杂志2018，Vol.27Issue(3)：157-163,7.DOI:10.11659/jjssx.12E017035
大鼠骨关节炎模型的建立及软骨组织中MMP-13和ADAMTS-5的表达
Establishment of rat osteoarthritis model and the expression of MMP-13 and ADAMTS-5 in cartilage tissue
摘要
Abstract
Objective To evaluate the feasibility of making the osteoarthritis (OA) model in the medial collateral ligament and the medial meniscus excision in rats,and to explore the mechanism of MMP-13 and ADAMTS-5 protein in the cartilage of rat osteoarthritis models.Methods Forty SD rats were randomly divided into model group(n =30) and sham operation group(n =10).The knee joint OA model was made from the medial collateral ligament of the knee and the medial meniscus,and the sham operation group was sutured after opening the capsule of the knee joint.Rats in the model group were killed at 4,6,and 8 weeks after the operation,and the sham operation group died of the rats at 8 weeks after the operation.The articular cartilage tissue of rats was taken.The expression level of MMP-13 and ADAMTS-5 protein in cartilage tissue was detected by Western blot and immunohistochemistry.Results Cartilage degeneration was observed in the model group 4 weeks after operation,and degeneration was further aggravated at 6 and 8 weeks.There was no significant degeneration in the sham operation group.There was a significant difference in the articular cartilage score between the two groups (P ＜ 0.05).The results of Western blot detection showed that the protein expression of MMP-13 and ADAMTS-5 was low in the sham operation group.The MMP-13 model began to rise for 4 weeks,and continued to rise in the 6th week,and the 8th week was lower than that of the previous one.The protein expression had significant difference in all groups at 4 weeks,6 weeks and 8 weeks (P ＜ 0.05).The ADAMTS-5 model began to rise for 6 weeks,and the expression level was maintained before the model was maintained for 8 weeks.The protein expression at 4 weeks compared to that at 6 weeks and 8 weeks had significant difference(P ＜0.05).The protein expression in rat articular cartilage tissue at 6 weeks and 8 weeks had no significant difference (P ＞ 0.05).The expression trend of immunohistochemical detection protein was consistent with that of Western blot.Conclusion The animal model of OA can be established by using the method of medial collateral ligament dissection and medial meniscectomy.The expression level of MMP-13 and ADAMTS-5 in different stages of OA has changed significantly.Further research is needed to explore its related signaling pathways.关键词
骨关节炎/基质金属蛋白酶/关节软骨/动物模型Key words
osteoarthritis/matrix metalloproteinase/articular cartilage/animal model分类
医药卫生引用本文复制引用
祁雷,姚运峰,李子煜,吴晗,荆珏华..大鼠骨关节炎模型的建立及软骨组织中MMP-13和ADAMTS-5的表达[J].局解手术学杂志,2018,27(3):157-163,7.基金项目
安徽省自然科学基金(1608085MH167) （1608085MH167）
安徽医科大学第二附属医院博士科研启动基金(2012BKJ014) （2012BKJ014）
