局解手术学杂志2018，Vol.27Issue(3)：163-167,5.DOI:10.11659/jjssx.11E017061
IL-6诱导鼻咽癌CNE-2细胞分泌IL-10的机制研究
Mechanism of IL-6 induced secretion of IL-10 in nasopharyngeal carcinoma CNE-2 cells
喻望博 1潘庆春 1李四军 1李婉 1毛丽芳2
作者信息
- 1. 川北医学院附属医院耳鼻咽喉科,四川南充637000
- 2. 川北医学院附属医院肿瘤科,四川南充637000
- 折叠
摘要
Abstract
Objective To study the mechanism of IL-6 stimulated the secretion of IL-10 in nasopharyngeal carcinoma CNE-2 cells.Methods Nasopharyngeal carcinoma CNE-2 cells were cultured with recombination cytokine IL-6 in vitro,or anti-IL-6 receptor antibody and signal pathway inhibitor were pre-incubated for 1 hour,and then IL-6 were added,the mRNA and protein level of IL-10 were separately detected by RT-PCR and enzyme-linked immunosorbent assay.Results Compared with the control group,the expression and secretion of IL-10 in IL-6 stimulated group were significantly increased,which was in a dose and time dependent way,the difference was significant(P ＜ 0.01).Additionally,IL-6 stimulated the expression and secretion of IL-10 by CNE-2 cells were significantly decreased in following pre-incubated with anti-IL-6 receptor antibody or NF-κB inhibitor,the difference was significant(P ＜ 0.01),but such effect was not detected when CNE-2 cells were pre-incubated with the PI3K inhibitor,p38/MAPK inhibitor,JNK inhibitor,MEK1/2 inhibitor and STAT3 inhibitor.Conclusion IL-6 can induce the expression and secretion of IL-10 in nasopharyngeal carcinoma CNE-2 cells via IL-6R/NF-κB signal pathway,and blocking IL-6 signal may be useful for the immunotherapy of nasopharyngeal carcinoma.关键词
白细胞介素-6/白细胞介素-10/NF-κB/鼻咽癌Key words
interlukin-6/interlukin-10/NF-κB/nasopharyngeal carcinoma分类
医药卫生引用本文复制引用
喻望博,潘庆春,李四军,李婉,毛丽芳..IL-6诱导鼻咽癌CNE-2细胞分泌IL-10的机制研究[J].局解手术学杂志,2018,27(3):163-167,5.
