局解手术学杂志2018，Vol.27Issue(3)：168-172,5.DOI:10.11659/jjssx.08E017001
人参皂苷Rg1对白血病干细胞衰老的影响
Effect of ginsenoside Rg1 on senescence characteristics of leukemic stem cells
摘要
Abstract
Objective To explore the effect of ginsenoside Rg1 on leukemia stem cells through comparing the biological senescence characteristics of HSCs in the patients with leukemia and healthy people,and provide new ideas and methods for leukemia prevention and treatment.Methods Fifteen cases of normal bone marrow in normal group and sixteen cases of chronic myeloid leukemia in leukemia group were divided into control group and Rg1 group,respectively.The control group used the conventional culture.The Rg1 group used the culture system with 10 μg/mL ginsenoside Rg1,other conditions were the same as control group.The bone marrow mononuclear cell of all groups were extracted after 2 days,and the CD34 +/CD38-cells population was isolated and purified by immunomagnetic adsorption cell sorting(MACS).The purity of the cells and cell cycles phase were detected by flow cytometry.Cell viability was detected by trypan blue staining.The percentage of positive cells was detected by SA-β-gal staining.CCK-8 detected the CD34 +/CD38-proliferation ability of each group.Results The ratio of CD34 +/CD38-cell population was (1.76 ± 0.34) ％ in every 1 × 106 BMNCs before sorting;the proportion of CD34 +/CD38-cell population per 1 × 106 cells after immunomagnetic sorting was (91.15 ± 2.41)％.The positive rate of SA-β-gal staining in human bone marrow CD34 +/CD38-cells of leukemia Rg1 group was significantly higher than that in leukemia control group,the difference was not significant (P ＞ 0.05);meanwhile there was no significant difference between normal control group and normal Rg1 group,but higher than that in leukemia control group,the difference was significant(P ＜ 0.05).CCK-8 results showed that the proliferation of CD34 +/CD38-cells was significantly increased in leukemia control group than those in the other groups.The survival rate of CD34 +/CD38-cells in human bone marrow was 99.1％ in all groups.Cell cycle phase results showed that the G1 arrest of CD34 +/CD38-cells in leukemia control group was significantly lower than those in the other three groups.Conclusion CD34 +/CD38-cells in chronic myeloid leukemia patients may be caused by some chronic myeloid leukemia.Ginsenoside Rg1 can effectively delay the process of aging.关键词
CD34+/CD38-造血干/祖细胞/人参皂苷Rg1/CCK-8/慢性髓细胞白血病/细胞周期时相Key words
CD34 +/CD38-hematopoietic stem/progenitor cells/ginsenoside Rg1/CCK-8/chronic myeloid leukemia/cell cycle phase分类
医药卫生引用本文复制引用
耿珊,陈雄斌,湛斌,季遥鹰,谢宝芬..人参皂苷Rg1对白血病干细胞衰老的影响[J].局解手术学杂志,2018,27(3):168-172,5.基金项目
重庆市卫生计生委医学科研计划项目(20143021) （20143021）
