局解手术学杂志2018，Vol.27Issue(3)：173-178,6.DOI:10.11659/jjssx.08E017020
低氧微环境对非小细胞肺癌细胞系A549细胞增殖和侵袭的影响
Effect of hypoxia on proliferation and invasion of non-small-cell lung cancer cell line A549
杨帆 1张超 2曾广大 2王剑 1郭道远 1熊玮1
作者信息
- 1. 陆军军医大学(第三军医大学)第一附属医院老年科,重庆400038
- 2. 陆军军医大学(第三军医大学)第一附属医院全科医学科,重庆400038
- 折叠
摘要
Abstract
Objective To study the effect of hypoxia on proliferation and invasion in non-small-cell lung cancer cell line A549 and its molecule mechanism.Methods The non-small-cell lung cancer cell line A549 were stimulated under hypoxia and normoxia for 24 hours in vitro.The proliferation ability was detected by CCK8.The invasion ability was detected by Transwell assay.The mRNA and protein expression levels of HIF-1 oα and β-catenin were detected by RT-PCR and Western blot.Furthermore,β-catenin siRNA was used to investigate the role of β-catenin on hypoxia induced proliferation and invasion.Results The results demonstrated that hypoxia could promote A549 cells proliferation and invasion.The mRNA and protein level of HIF-1oα and β-catenin was upregulated by hypoxia treatment,whereas β-catenin siRNA could block these processes.Conclusion Hypoxia can promote the proliferation and invasion of non-small-cell lung cancer cell line A549 by activating Wnt/β-catenin signaling pathway.关键词
肺癌/低氧/B-连环蛋白/增殖/侵袭Key words
lung cancer/hypoxia/Wnt/β-catenin/proliferation/invasion分类
医药卫生引用本文复制引用
杨帆,张超,曾广大,王剑,郭道远,熊玮..低氧微环境对非小细胞肺癌细胞系A549细胞增殖和侵袭的影响[J].局解手术学杂志,2018,27(3):173-178,6.
