中国水产科学2018，Vol.25Issue(3)：467-474,8.DOI:10.3724/SP.J.1118.2018.17252
基于心电图法(ECG)的牙鲆听觉特性研究
Study on hearing capacities of Paralichthys olivaceus using an ECG method
摘要
Abstract
In recent years, as fishery resources have dwindled, the hearing capacities of fish have been studied in ambient noise assessments, for the protection of fish, and also in the construction of marine ranching by using the acoustic domestication method. The use of an electrocardiogram (ECG) offers a method for the study of fish hearing capacities. The method comprises behavioral and electrophysiology sciences and is based on a conditioned reflex reaction to acoustic and electrical stimulus to monitor the hearing threshold through the heart rate of fish. Paralichthys olivaceus, a bottom-living fish without a swim bladder, is predominantly a commercial species in northern China. The auditory sensor organs of P. olivaceus are the otolith and the lateral line. The objective of this study was to use an ECG method to measure the hearing threshold of P. olivaceus, by using self-established fish auditory sensation measuring devices, to provide a reference for the development and construction of marine ranching in China. The following acoustic stimulus frequencies were selected in the study: 60 Hz, 100 Hz, 150 Hz, 200 Hz, 300 Hz, 400 Hz, 500 Hz, 700 Hz and used to measure the hearing threshold of 20 independent fish, from which audiograms could be drawn and analyzed. Before the experiment, an acoustic and electoral stimulus condi-tioned reflex in the fish was established to ensure the fish could develop memories of the stimulus, and then the hearing threshold was estimated by monitoring the heart rate of the fish, which was recorded by using a silver wire inserted in the fish heart. The ECG signals from 20 ind fish were obtained and the results were as follows: the hearing sensitive frequency bandwidth of P. olivaceus was between 60 Hz and 200 Hz and the hearing threshold was below 136 dB (re: 1 μPa); 100 Hz was the most sensitive frequency bandwidth and the hearing threshold was 94±1.5 dB. The advantages of ECG method were that a stable signal was obtained, the subjected fish show an ob-vious conditioned response to the acoustic stimulus, the quick recovery allowed repeated testing. The hearing threshold of P. olivaceus is similar to Limanda limanda, which have same body shape; they are sensitive to acous-tic stimuli with a frequency of 60–200 Hz. The results of the study could help to promote fundamental research in fish auditory sensation and provide a reference for the construction of marine ranching and the future development of new tapes for fishing nets by using acoustic methods.关键词
心电图/牙鲆/听觉阈值/电刺激Key words
electrocardiogram/Paralichthys olivacues/hearing threshold/electrical stimulus分类
农业科技引用本文复制引用
邢彬彬,许柳雄,王振宇,张国胜,庄鑫,殷雷明,王羿宁,李泓泉,刘景,刘宏超..基于心电图法(ECG)的牙鲆听觉特性研究[J].中国水产科学,2018,25(3):467-474,8.基金项目
国家科技支撑计划项目(2013BAD23B02) （2013BAD23B02）
农业部北方海水增养殖重点实验室开放课题(2014-MSENC-KF-13). （2014-MSENC-KF-13）
