中国水产科学2018，Vol.25Issue(3)：503-511,9.DOI:10.3724/SP.J.1118.2018.17428
养殖金乌贼卵子发生和卵巢发育的组织学观察
Histology of oogenesis and ovarian development in cultured Sepia esculenta
摘要
Abstract
The golden cuttlefish (Sepia esculenta Hoyle) is one of the most important commercial species in China and Japan. In order to protect and restore cuttlefish resources, researchers have paid a lot of attention to the re-productive biology and artificial breeding techniques of cuttlefish. In the present study, both anatomic methods and tissue section techniques were used to describe the histological and morphological characteristics of oogenesis and ovary development in cuttlefish, in order to elucidate the reproductive physiology of golden cuttlefish. During the oogenesis of S. esculenta, follicular cells were always observed and underwent structural changes before oo-cyte maturation. The disintegration of follicular cells and the occurrence of yolk substances occurred synchro-nously. Egg yolk substances accumulated gradually until the oocytes were full and the follicular cells had disinte-grated completely, and the nuclei gradually disappeared along with the follicular cells. Eggs were not synchro-nized and exhibited significantly variable morphology. Immature oocytes adhered to the ovarian matrix and circu-lated around the matrix of the ovary so that oocytes closer to the center were less developed than those further away. After maturation, the oocytes were free in the ovarian cavity and were discharged through the oviduct. Ac-cording to the morphology of oocytes and follicular cells and vitellogenesis, the asynchronous oogenesis of S. esculenta could be categorized into five stages, with nine phases: proliferative stage (oogonia phase), small growth stage (free follicular cell phase, single follicular cell phase, and double follicular cell phase), large growth stage (follicle penetration phase, pre-vitellogenic phase, and late-vitellogenic phase), maturation stage, and resorbing stage. Meanwhile, the ovarian development cycle could be categorized into six stages (I–VI), according to ovary appearance and gonad index (GI) and dominant phase of oocytes. Understanding the development and oogenesis of S. esculenta is of great significance to the theory of cephalopod reproductive biology and improving artificial propagation technology.关键词
金乌贼/卵子发生时相/卵巢发育分期/组织学特征/解剖学特征Key words
Sepia esculenta Hoyle/oogenesis/ovarian development/histological characteristic/anatomy characteristic分类
农业科技引用本文复制引用
尹亚南,刘长琳,胡鹏,张金勇,柳淑芳,庄志猛,薛同明..养殖金乌贼卵子发生和卵巢发育的组织学观察[J].中国水产科学,2018,25(3):503-511,9.基金项目
国家自然科学基金项目(31672645) （31672645）
中央级公益性科研院所基本科研业务费专项(20603022016001) （20603022016001）
山东省泰山学者建设工程专项. （）
