中国水产科学2018，Vol.25Issue(3)：520-535,16.DOI:10.3724/SP.J.1118.2018.17293
三疣梭子蟹氧化磷酸化代谢在家系近交中的变化
Effects of inbreeding on the oxidative phosphorylation of the swim-ming crab Portunus trituberculatus
摘要
Abstract
The swimming crab Portunus trituberculatus (Crustacea: Decapoda: Brachyura) is a dominant species of portunid crab fisheries and an important economic species worldwide. The key performance traits of crustaceans and other aquaculture species are often depressed by inbreeding, especially if inbreeding effects accumulate too rapidly. Although previous studies have investigated the effects of inbreeding on the morphology, hatchability, and survival of crustaceans, little evidence is available that demonstrates that inbreeding affects crustacean physiology. Therefore, in order to investigate the effects of inbreeding on the oxidative phosphorylation of P. trituberculatus, cDNAs of the key subunit genes of the four mitochondrial respiratory chain complexes were cloned, sequenced using rapid amplification of cDNA ends (RACE), and then analyzed using bioinformatics technology. The full-length cDNA sequence of the Complex Ⅰ core subunit genes was 1005 bp in length and contained a 705-bp open reading frame (ORF) that encoded a 234-amino acid polypeptide (GenBank: KY682717). The full-length cDNA sequence of Complex Ⅱ was 915 bp in length and contained a 540-bp ORF that encoded a 179-amino acid polypeptide (GenBank: KY406169). The full-length cDNA sequence of the Complex Ⅲ subunit (Cytc1) gene was 2371 bp in length and contained a 942-bp ORF that encoded a 313-amino acid polypeptide (GenBank: KY406171), and the full-length cDNA for the key subunit of Complex IV was 1171 bp in length and encoded 105 amino acids (GenBank: KY406170). In addition, homology and phylogenetic analyses revealed that the amino acid sequences of the four complexes were highly similar to those of closely related species and had higher conservation in evo-lution, and could be used as a reference for other marine organisms. The activities and mRNA expression of the four complexes in the hepatopancreas and heart mitochondria of P. trituberculatus were investigated. Results show that inbreeding reduced the activity of all four complexes and their respective subunit gene in the hepatopancreas (P<0.05). Besides, Complex I, III and IV activities and their subunit genes in heart were declined by inbreeding (P<0.05). Furthermore, the elevated activity and expression of Complex II in the heart may indicate that the ability to oxidize succinic acid and the level of aerobic metabolism in the crabs rose by breeding might because the dominant homozygous genes were accumulated during the family-based selective breeding programs. Therefore, it is clear that inbreeding gradually reduces the oxidative phosphorylation pathway and provides a reference for family-based selective breeding programs for P. trituberculatus.关键词
三疣梭子蟹/氧化磷酸化代谢/基因克隆/复合体/近交衰退Key words
Portunus trituberculatus/phosphorylation/gene clone/complex/inbreeding depression分类
农业科技引用本文复制引用
王竹青,任宪云,高保全,刘萍,李健,王磊..三疣梭子蟹氧化磷酸化代谢在家系近交中的变化[J].中国水产科学,2018,25(3):520-535,16.基金项目
国家自然科学基金项目(41576147, 41506186) （41576147, 41506186）
泰山领军人才工程高效生态农业创新类计划项目(LJNY2015002) （LJNY2015002）
国家虾蟹产业技术体系项目(CARS-48). （CARS-48）
