中国现代医学杂志2018，Vol.28Issue(15)：1-6,6.DOI:10.3969/j.issn.1005-8982.2018.15.001
ATG5介导肝癌细胞对抗营养危机的作用研究
Effect of ATG5-mediated anti-nutritional crisis in hepatocellular carcinoma
摘要
Abstract
Objective To investigate the effect of anti-nutritional crisis in tumor cells and potential molecular mechanisms. Methods Normal mouse liver cell AML-12 as well as hepatocellular carcinoma cell line Hepa1-6 was utilized in this study. ATG5 expressing construct was transfected into cells. Si-RNA technology was performed for silencing of targeted genes. Quantitative real-time reverse transcription PCR (qRT-PCR) analysis and Western blot were carried for RNA levels and proteins, respectively. Cell cycle status and cellular activity were identified by flow cytometry and CCK-8 kit, respectively. Results Hepa1-6 was more resistant to nutrition deprivation compared with AML-12. Serum withdrawal induced remarkable increased expression of ATG5 in Hepa1-6 cells (P < 0.05). Knockdown of ATG5 reversed resistance of cells to nutrition deprivation. Further, ATG5 induced increase of p21 (cip1/waf1), which rested the Hepa1-6 cells in G1 phase. Conclusion Resistance of tumor cells to nutrient deficiency may be achieved through ATG5-p21 (cip1/waf1) signaling pathway which rests cells on G1 stage.关键词
缺血/细胞死亡/自噬/ATG5/p21(cip1/waf1)Key words
serum withdrawal/cell death/autophagy/ATG5/p21 (cip1/waf1)分类
医药卫生引用本文复制引用
马成,马孙强,李济宇..ATG5介导肝癌细胞对抗营养危机的作用研究[J].中国现代医学杂志,2018,28(15):1-6,6.基金项目
国家自然科学基金(No:81470897) （No:81470897）
