中国现代医学杂志2018，Vol.28Issue(16)：6-10,5.DOI:10.3969/j.issn.1005-8982.2018.16.002
胸腺素α1对重症急性胰腺炎大鼠肺损伤作用的实验研究
Experimental study about effect of thymosin alpha l on lung injury in rats with severe acute pancreatitis
摘要
Abstract
Objective To explore the protective effect of thymosin alpha l on lung injury in rats with severe acute pancreatitis (SAP) and its possible mechanism. Methods Twenty-four adult male Sprague-Dawley rats were randomly divided into three groups: control group (A), severe acute pancreatitis group (B) and thymosin alpha l intervention group (C) with 8 in each group. Animal models of SAP were maken by retrograde injection of 5％sodium taurocholate (0.1 ml/100 g) into the cholangiopancreatic duct. The rats in the group C were treated with thymosin α1 (6 mg/kg) by subcutaneous administration 30 min prior to SAP modeling. The rats from each group were sacrificed 12 h after SAP modeling. The selum level of amylase and lung W/D ratio were detected in each group. Intercelluar adhesion molecule 1 (ICAM-1) and tumor necrosis factor-α (TNF-α) levels were studied using Western blot. Histopathological changes of pancreatic and pulmonary tissues were observed by light microscopy. ResultsThe levels of serum amylase, the expressions of ICAM-1 and TNF-α in the lungs, the lung W/D ratio, and the lung and pancreas pathologic scores were increased in the groups B and C compared with the group A (P < 0.05). The levels of serum amylase, the expressions of ICAM-1 and TNF-α in the lungs, the lung W/D ratio, and the lung and pancreas pathological scores in the group C were much lower than those in the group B (P < 0.05). The changes of histopathology were milder in the group C. There was no obvious pathological changes in the group A. Conclusions Thymosin α 1 can decrease pulmonary edema, reduce pulmonary injury and has protective effect on lung injury in the rats with SAP by reducing the expressions of TNF-α and ICAM-1.关键词
胸腺素α1/重症急性胰腺炎/肺损伤/肿瘤坏死因子αKey words
thymosin α1/severe acute pancreatitis/lung injury/tumor necrosis factor-α分类
医药卫生引用本文复制引用
邱兆磊,王振杰,程峰,郑传明,李磊,姜海,杜召辉..胸腺素α1对重症急性胰腺炎大鼠肺损伤作用的实验研究[J].中国现代医学杂志,2018,28(16):6-10,5.基金项目
安徽省教育厅自然科学资助项目(No:KJ2015B113by) （No:KJ2015B113by）
安徽省蚌埠医学院科技发展资助项目(No:BYKF1768) （No:BYKF1768）
