摘要
Abstract
Objective To investigate the effect of Sufentanil postconditioning on Nrf2-ARE signaling pathway during myocardial ischemia-reperfusion injury of rats. Methods Thirty healthy male SD rats were randomly divided into sham-operation group, ischemia-reperfusion group and Sufentanil postconditioning group. The rat models of myocardial ischemia-reperfusion injury were prepared. In the Sufentanil postconditioning group, Sufentanil was injected into femoral vein 5 min before reperfusion at a dose of 1 μg/kg, the rats in the sham operation group and the ischemia-reperfusion group were injected with the same amount of normal saline. The myocardial infarct sizes and pathologic changes in three groups were detected. The cardiomyocyte apoptosis in the three groups was detected. The expressions of Nrf2,HO-1,NQO1,SOD1 and GSTM2 genes and proteins were detected by qRT-PCR and Western blot, respectively. Results Compared with the sham-operation group, the cell apoptotic indexes and the myocardial infarct sizes in the ischemia-reperfusion group and the Sufentanil postconditioning group were increased (P < 0.05). Compared with the ischemia-reperfusion group, the cell apoptotic index and the myocardial infarct size in the Sufentanil postconditioning group were significantly decreased (P < 0.05). Compared with the sham-operation group, the relative expression levels ofNrf2,HO-1,NQO1,SOD1 andGSTM2 genes and proteins in the ischemia-reperfusion group and the Sufentanil postconditioning group were significantly decreased (P < 0.05). Compared with the ischemia-reperfusion group, the relative expression levels ofNrf2,HO-1,NQO1,SOD1 andGSTM2 genes and proteins in the Sufentanil postconditioning group were increased, the differences were statistically significant (P < 0.05). Conclusions Sufentanil postconditioning could effectively reduce the myocardial infarct size and cell apoptosis in the rats with ischemia-reperfusion injury. The mechanism might be through the activation of Nrf2/ARE signaling pathway to promote the expressions of the downstream phase II detoxification enzymes and antioxidant enzymes, and thus effectively resist oxidative stress injury.关键词
舒芬太尼/缺血后处理/心肌缺血再灌注损伤/Nrf2-ARE信号通路/大鼠Key words
Sufentanil/ischemic postconditioning/myocardial ischemia-reperfusion injury/Nrf2-ARE signaling pathway/rat分类
医药卫生
