摘要
Abstract
ObjectiveTo investigate the targeting of a novel radionuclide molecular probe18F-NT in prostate cancer cells and tumor bearing mice, and to provide experimental evidence forin vivo imaging of 18F-NT targeted prostate cancer. Methods18F-NT was prepared, and quality control testing was completed. A human prostate cancer cell line PC3 with high expression of neurotensin receptor 1 (NTR1) was selected. PC3 cells were divided into three groups. The control group received free18F ions into the cells. In the experimental group,18F-NT was added to the cells. Neurotensin (NT) was added to the blocking group 30 min before addition of18F-NT. Nude mice were divided into two groups (experimental group and blocking group), each group had 3 mice. In the blocking group, 0.2 ml of 1.0 mg/ml NT was injected into the tail vein of each nude mouse, and each mouse in the experimental group was injected with 0.2 ml of saline. After 30 min, each nude mouse in the two groups was injected with 0.2 ml18F-NT (the radioactive concentration was 37 MBq/ml). After 1 h, the nude mice were sacrificed and the major organs and the tumor tissues were separated. γ counter was used to measure the radioactive count of the organs and the tissues. The radioactive count of PC3 cells and radioactive uptake values (％ ID/g) of the tumor tissues were analyzed statistically. Results The18F-NT was successfully prepared, its physical and chemical properties and the quality control indicators reached standard. Cell-binding assay showed that the radioactive count of the experimental group [(5,825.00 ± 1,074.52)/min] was significantly higher than that of the blocking group [(1,941.66 ± 173.58)/min], the difference was statistically significant (t = 7.227,P = 0.003), while that of the free18F control group was only (170.33 ± 56.59)/min. Thein vivo biological distribution experiment of the two groups of nude mice showed that blood removal rate of18F-NT was fast. The18F-NT was mainly metabolized by the kidneys. The radioactive uptake value of the tumor tissues was the highest in the experimental group [(1.02 ± 0.49)％ ID/g], while it was significantly lower in the blocking group [(0.21 ± 0.03)％ ID/g], the difference was statistically significant (t = 2.815,P = 0.049). Conclusions The18F-NT has high labeling rate and radioactive chemical purity, and also excellentin vitro stability. The uptake of18F-NT is very high in both human prostate cancer cell line PC3 and PC3 tumor-bearing nude mice, which could be effectively blocked by NT. This experiment can lay a good foundation for the follow-up targeting NTR1in vivo imaging.关键词
18F-NT/前列腺癌/PC3/细胞结合实验/生物学分布实验Key words
18F-NT/prostate cancer/PC3/cell binding experiment/biological distribution experiment分类
医药卫生
