中国现代医学杂志2018，Vol.28Issue(16)：22-28,7.DOI:10.3969/j.issn.1005-8982.2018.16.005
宫颈癌细胞系中HPV感染与GRP78、JNK及CHOP表达的相关性分析
Correlations of HPV infection with GRP78, JNK and CHOP expressions in cervical cancer cell lines
摘要
Abstract
Objective To investigate the correlations of HPV infection with the expressions of endoplasmic reticulum stress related proteins in cervical cancer cell lines. Methods Immunocytochemistry, Western blot and reverse transcription polymerase chain reaction (RT-PCR) were employed to detect the protein and mRNA expression levels of glucose-regulated protein 78 (GRP78), C-Jun NH2-terminal kinase (JNK) and CCAAT/enhancer-binding protein homologous protein (CHOP) in HPV16-positive SiHa, HPV18-positive Hela and HPV-negative C33a cervical cancer cell lines. Statistical methods were applied to analyze the statistical differences of their expressions. Results The results of immunocytochemistry showed that GRP78 protein levels of high-risk HPV16-positive SiHa and HPV18-positive Hela cells were significantly higher than that of HPV-negative C33a (P < 0.05), and endoplasmic reticulum stress related proteins JNK and CHOP also had notably higher expressions in high-risk HPV16-positive SiHa and HPV18-positive Hela cells than in the HPV-negative C33a cells (P < 0.05). RT-PCR and Western blot results showed that the mRNA levels of GRP78, JNK and CHOP in HPV16-positive SiHa cells and HPV18-positive Hela cells were significantly higher than those in HPV-negative C33a cells (P < 0.05). Conclusions HPV subtypes are strongly associated with the expressions of GRP78 and endoplasmic reticulum stress related proteins JNK and CHOP, and endoplasmic reticulum stress related proteins may be involved in the occurrence and development of cervical cancer.关键词
宫颈癌/人乳头瘤病毒/内质网应激蛋白Key words
cervical cancer/human papilloma virus (HPV)/endoplasmic reticulum stress related proteins分类
医药卫生引用本文复制引用
刘卿,郭兵,秦娟..宫颈癌细胞系中HPV感染与GRP78、JNK及CHOP表达的相关性分析[J].中国现代医学杂志,2018,28(16):22-28,7.基金项目
国家自然科学基金(No:NS2015-36) （No:NS2015-36）
