中国现代医学杂志2018，Vol.28Issue(16)：34-37,4.DOI:10.3969/j.issn.1005-8982.2018.16.007
牡荆素对铜绿假单胞菌生物膜形成的影响
Anti-biofilm effect of vitexin combined with antibiotics on Pseudomonas aeruginosa
蒙传任 1林稚凤 1邢红宇2
作者信息
- 1. 海南省海口市第四人民医院 检验科,海南 海口 571100
- 2. 海南省中医院 检验科,海南 海口 570311
- 折叠
摘要
Abstract
Objective To explore the anti-biofilm effect of vitexin combined with antibiotics onPseudomonas aeruginosa. MethodsMinimal inhibitory concentrations (MICs) of antibiotics toP.aeruginosa were determined by broth microdilution method. And 96-well cell culture plate combined with crystal violet was used to study anti-biofilm effect of vitexin alone or combined with antibiotics. Results Vitexin could significantly inhibitP. aeruginosa biofilm formation from 100.00％ to (80.00 ± 4.62) ％ at the concentration≥ 31.25 μg/ml in a dose-dependent manner (P < 0.05). The MICs of CAZ, CIP, GEN and OFLX were 0.5, 0.5, 2.0 and 8.0 μg/ml, respectively. And vitexin showed synergistic inhibition of PAO1 biofilm formation when combined with sub-MIC of CAZ, CIP, GEN and OFLX, which could reduce the total amount of biofilm from 100.00％ to (39.38 ± 5.32) ％, (40.31 ± 2.95) ％, (23.31 ± 3.95) ％ and (50.23 ± 3.14) ％, respectively (P < 0.05). Conclusions Vitexin significantly inhibitsbiofilm formation ofP.aeruginosa, and shows synergistic effect when combined with antibiotics.关键词
牡荆素/铜绿假单胞菌/生物膜/联合用药/协同作用Key words
vitexin/Pseudomonas aeruginosa/biofilm/drug combination/synergistic effect分类
医药卫生引用本文复制引用
蒙传任,林稚凤,邢红宇..牡荆素对铜绿假单胞菌生物膜形成的影响[J].中国现代医学杂志,2018,28(16):34-37,4.
