中国药房2018，Vol.29Issue(11)：1483-1487,5.DOI:10.6039/j.issn.1001-0408.2018.11.11
头花蓼内生真菌Aspergillus terreus油脂类代谢物的鉴定及其抗多药耐药菌和抗炎作用研究
Study on Identification of Lipid Metabolites of Endophytic Fungus Aspergillus terreus from Polygonum capitatum and Their Anti-multidrug Resistant Bacteria and Anti-inflammatory Effects
摘要
Abstract
OBJECTIVE:To identify lipid metabolites of endophytic fungus Aspergillus terreus from Polygonum capitatum, and to investigate their anti-multidrug resistant bacteria and anti-inflammatory effects. METHODS:GC-MS was used to analyze and identify lipid metabolites of A. terreus. MIC of lipid metabolites and main composition to 10 strains of multidrug resistant bacteria (Klebsiella pneumoniae,Proteus common,Staphylococcus epidermidis,Escherichia coli,Staphylococcus aureus,Enterobacter cloacae,Pseudomonas aeruginosa,Proteus mirabilis,Enterococcus faecium and Acinetobacter baumanii) were determined by 96-well plate microdilution method. The spread plate method was used to determine MBC of samples to bacteria. LPS-induced RAW264.7 inflammatory model was used to investigate the effects of different mass concentrations(50,100,200 μg/mL)of lipid metabolites on the release of NO and TNF-α after treated for 24 h. RESULTS:A total of 13 compounds were identified in lipid metabolites of A. terreus,among which palmitic acid,stearic acid,linoleic acid and oleic acid were main components,and relative percentages of them were 29.35％,10.87％,21.94％,34.85％. The lipid metabolites displayed anti-bacterial activity against E. coli and K. pneumonia with MICs of 12.5 mg/mL and 50 mg/mL,MBC of 25 mg/mL and 100 mg/mL,respectively. The main 4 compositions could inhibit the growth of E. coli,with MIC of 0.5-1 mg/mL,among which palmitic acid showed significant antibacterial activity,especially to E. faecium(MIC of 0.25 mg/mL). 50,100 μg/mL lipid metabolites could signifiantly inhibit the release of inflammatory factor of NO and TNF-α in RAW264.7 in vitro. CONCLUSIONS:The lipid metabolites of endophytic fungus A. terreus from P. capitatum show anti- multi-drug resistant bacteria and anti-inflammatory effects.关键词
头花蓼/内生真菌Aspergillusterreus/油脂类代谢物/抗多药耐药菌活性/抗炎作用Key words
Polygonum capitatum/Endophytic fungus Aspergillus terreus/Lipid metabolites/Anti-multidrug resistant bacteria activity/Anti-inflammatory effect分类
化学化工引用本文复制引用
刘俊,张青艳,杨馨,周孟,廖尚高,徐国波..头花蓼内生真菌Aspergillus terreus油脂类代谢物的鉴定及其抗多药耐药菌和抗炎作用研究[J].中国药房,2018,29(11):1483-1487,5.基金项目
国家自然科学基金资助项目(No.81560570) （No.81560570）
贵州省教育厅自然科学研究项目(No.黔教合KY字〔2015〕441号) （No.黔教合KY字〔2015〕441号）
