中国药理学通报2018，Vol.34Issue(6)：764-769,6.DOI:10.3969/j.issn.1001-1978.2018.06.007
荭草花中五种成分在Caco-2细胞中的吸收特性研究
Study on absorption activity of five components of Polygonum orientale L. Flower in Caco-2 cell monolayer
摘要
Abstract
Aim To observe the absorption activity of five components of Polygonum orientale L. Flower ex-tract in Caco-2 cell monolayer. Methods The effects of different concentrations, time, temperature, pH and P-glycoprotein inhibitors on Caco-2 cells were investi-gated by UPLC-MS/MS. Results The absorption of five components of Polygonum orientale L. Flower ex-tract presented a concentration-and time-dependent manner, and the uptake of quercetin was reduced in Caco-2 cells after 90 min. There was a highest intake at 37℃,and the uptake of four components was best in acid environment except for the quercetin at pH 6 , which was best at pH5 . The uptake of quercetin and kaempferol was significantly improved after the addition of P-glycoprotein inhibitors of verapamil. Conclusions The cellular uptake mechanisms of the five compo-nents of Polygonum orientale L. Flower extract is man-inly through passive diffusion. P-glycoprotein is in-volved in the uptake of quercetin and kaempferol.关键词
荭草花提取物/Caco-2细胞/P-糖蛋白抑制剂/吸收机制/UPLC-MS/MS/摄取Key words
Polygonum orientale L. Flower extract/Caco-2 cell/P-glycoprotein inhibitors/absorption mechanism/UPLC-MS/MS/uptake分类
医药卫生引用本文复制引用
陈浩,覃小丽,夏涛,李月婷,巩仔鹏,李勇军,郑林..荭草花中五种成分在Caco-2细胞中的吸收特性研究[J].中国药理学通报,2018,34(6):764-769,6.基金项目
国家自然科学基金资助项目(No 81360664,81560630) （No 81360664,81560630）
贵州省科技计划项目(黔科合平台人才[2017]5601,[2016] 5613/5677) （黔科合平台人才[2017]5601,[2016] 5613/5677）
贵阳市科技局项目(筑科合同[2017]30-29号) （筑科合同[2017]30-29号）
