中国药理学通报2018，Vol.34Issue(6)：820-824,5.DOI:10.3969/j.issn.1001-1978.2018.06.016
黄芩素对大鼠胰岛素分泌的作用及机制研究
Effect of baicalein on pancreas insulin secretion in rats and its mechanism
摘要
Abstract
Aim To investigate the effect of baicalein on insulin secretion from rat islets and the underlying mechanism. Methods Pancreatic islets were obtained from the pancreas of male Wistar rats by collagenase P digestion and histopaque-1077 density gradient separa-tion. Single islet cells were dispersed from pancreatic islets by Dispase II digestion. Insulin secretion experi-ment was applied to observe insulin release after baica-lein stimulation. To study the potential mechanism, calcium imaging technique and patch-clamp experiment were applied to measure intracellular Ca2+ concentra-tion and voltage-dependent potassium channel currents (Kv). Results In 16. 7mmol·L-1 glucose, baica-lein accelerated insulin secretion in a dose-dependent manner. Baicalein promoted the intracellular Ca2+ con-centration. The patch-clamp experiment showed that baicalein inhibited Kv current in a dose-dependent manner. Conclusion Baicalein can increase the in-tracellular Ca2+ concentration by inhibiting Kv chan-nels and eventually promoting insulin secretion.关键词
黄芩素/胰岛/胰岛β细胞/胰岛素分泌/电压依赖型钾通道/钙成像Key words
baicalein/pancreatic islet/islet β cell/insulin secretion/voltage-dependent potassium chan-nels/calcium imaging分类
医药卫生引用本文复制引用
郭阳艳,刘萌萌,杨晓华,任乐乐,刘志宏,刘云峰,章毅..黄芩素对大鼠胰岛素分泌的作用及机制研究[J].中国药理学通报,2018,34(6):820-824,5.基金项目
国家自然科学基金资助项目( No 81670710,81770776, 81373464) （ No 81670710,81770776, 81373464）
山西省留学人员科技活动项目择优资助( No 2016-97) （ No 2016-97）
山西省回国留学人员科研资助项目( No 2017-053) （ No 2017-053）
山西省"1331 工程"重点学科建设计划经费资助 （）
山西省重点学科建设经费资助 （）
