中国药理学通报2018，Vol.34Issue(6)：831-836,6.DOI:10.3969/j.issn.1001-1978.2018.06.018
红景天苷对人脐静脉内皮细胞氧糖剥夺/复氧损伤的保护作用
The protective effect of salidroside on oxygen glucose deprivation/reoxygenation in human umbilical vein cells
摘要
Abstract
Aim To investigate the effects of salidro-side on oxygen glucose deprivation/reoxygenation ( OGD/R) injury in human umbilical vein cells ( HU-VECs ) and the possible mechanism. Methods OGD/R model was built after the density of cell growth reached 80％ ～85％. The OGD time point was deter-mined by MTT method to detect cell viability;LDH ac-tivity detection was used to explore the best dosage sol-ubility;NO content assay was applied to determine the best drug solubility, the degree of apoptosis was detec-ted by Annexin V/PI, and the expression of P-selectin was detected by immunohistochemistry. Results MTT detection determined that the time point of OGD was 16 h; LDH activity detection and NO content detection showed that the best efficacy of salidroside was 10μmol · L-1 . Compared with control group, Annexin V/PI detection showed that the apoptotic rate increased significantly ( P <0.01 ) after OGD/R, and the ex-pression of P-selectin in OGD/R group obviously in-creased ( P <0.01 ) . Compared with OGD/R group,the expression of P-selectin obviously declined ( P <0.05 ) , while the cell apoptotic rate increased ( P <0.05) after the administration. Conclusions Salidro-side may protect HUVEC cells from OGD/R injury and its mechanism may be related to related to the down-regulation of P-selectin and the inhibition of the pro-duction of inflammation.关键词
红景天苷/炎症反应/脑缺血/再灌注/氧糖剥夺/复氧/人脐静脉内皮细胞/P-selectinKey words
salidroside/inflammation/cerebral ische-mia/reperfusion/oxygen deprivation/reoxygenation/human umbilical vein endothelial cells/P-selectin分类
医药卫生引用本文复制引用
苏燕青,汪旭雯,王颖峥,宋百颖,林昱,黄鑫,洪桂祝..红景天苷对人脐静脉内皮细胞氧糖剥夺/复氧损伤的保护作用[J].中国药理学通报,2018,34(6):831-836,6.基金项目
国家自然科学基金资助项目(No 81473382) （No 81473382）
