中国药理学通报2018，Vol.34Issue(6)：846-850,5.DOI:10.3969/j.issn.1001-1978.2018.06.021
高糖对肾小球系膜细胞BKCa-Orai1信号复合物表达与功能的影响
Expression and function of Orai1-BK Ca complex in high glucose treatment rat glomerular mesangial cells
摘要
Abstract
Aim To investigate whether Orai1 and BKCa form a physical and functional signal complex in glomerular mesangial cells ( GMCs) and reveal the role of high glucose treatment on Orai1-BKCa complex and SOCE-induced hyperpolarization. Methods The in-teraction of Orai1 and BKCa was evaluated by co-immu-noprecipitation ( co-IP ) . Western blot method was used to detect the expression levels of Orai1 and BKCa . SOCE was activated by Ca2+ depletion evoked by TG in normal glucose and high glucose. The DiBAC4(3) was employed as fluorescence indicator to measure the potential change of membrane. . Results The co-IP experiment results showed that Orai1 interacted with BKCa in GMCs. SOCE induced the hyperpolarization of GMCs. SOCE and SOCE-induced membrane hyperpol-orizaiton were enhanced by high glucose treatment for three days. In addition, the expression levels of Orai1 and BKCa were enhanced significantly in the high glu-cose-cultured cells. Conclusions Orai1 can form a signaling complex with BKCa , which participates in the regulation of hyperpolarization in GMCs and may be in-volved in the hyperpolarization in high glucose cultured GMCs.关键词
肾小球系膜细胞/高糖/大电导钙激活钾通道/Orai1/钙库操纵钙内流/膜电位Key words
glomerular mesangial cells/high glucose/BKCa/Orai1/SOCE/membrane potential分类
医药卫生引用本文复制引用
杜鹃,闫德俊,杨云云,陈硕,魏圆,沈兵..高糖对肾小球系膜细胞BKCa-Orai1信号复合物表达与功能的影响[J].中国药理学通报,2018,34(6):846-850,5.基金项目
国家自然科学基金资助项目( No U1732157 ) （ No U1732157 ）
安徽省自然科学基金资助项目(No 1708085MH187) （No 1708085MH187）
安徽省高校优秀青年人才支持计划 （）
