中国药理学通报2018，Vol.34Issue(6)：873-878,6.DOI:10.3969/j.issn.1001-1978.2018.06.026
芪智消渴颗粒对MPTP诱导斑马鱼神经损伤的保护作用
The protective effect of QZXK in nerve injury using zebrafish model induced by MPTP
摘要
Abstract
Aim To investigate the protective effect of Qi ZhiXiaoke granules ( QZXK ) on nerve injury using zebrafish and nerve cell injury models. Methods The nerve injury model was established using wild zebrafish AB line, 72 hours after fertilization treated with 1-methyl-4-phenyl-1 , 2 , 3 , 6-four pyridine ( MPTP ) .Then QZXK of different doses were administered for three days,and the trajectory of the zebrafish behavior was recorded and analyzed. Neuroblastoma PC12 cells were incubated with different concentrations of QZXK and MPTP,and the cell viability of PC12 cells was de-tected by MTT. The mitochondrial membrane potential and expression of apoptosis related protein Caspase3 were measured by kits. Results Compared with con-trol group,MPTP reduced the movement distance of ze-brafish,and with the increase of concentration, QZXK promoted the movement distance and reversed the swimming behavior abnormality of zebrafish. Compared with control group, QZXK could inhibit the apoptosis induced by MPTP and promote the cell viability of PC12 cells with MPTP. QZXK improved the membranepotential and decreased the expression of Caspase3 . Conclusions QZXK exerts neuroprotective effect in the process of nerve injury induced by MPTP. The mechanism may be related with inhibiting apoptosis of neural cells. These experiment provides experimental and theoretical foundation for QZXK promoting cogni-tive function.关键词
芪智消渴颗粒/斑马鱼/MPTP/PC12细胞/神经保护/凋亡Key words
QZXK/zebrafish/MPTP/PC12 cells/nerve protection/apoptosis分类
医药卫生引用本文复制引用
王荣春,程丽芳,韩利文,何秋霞,陈锡强,侯海荣,张新军,李莹,刘可春..芪智消渴颗粒对MPTP诱导斑马鱼神经损伤的保护作用[J].中国药理学通报,2018,34(6):873-878,6.基金项目
山东省自然科学基金三院联合基金(No ZR2016YL009) （No ZR2016YL009）
山东省科学院青年基金项目(No 2015QN010) （No 2015QN010）
山东省科技发展计划项目(No 2014GSF119009) （No 2014GSF119009）
